Repeated batch fermentation for protease production using biofilm fermentation of Bacillus sp.

Repeated batch fermentation is a stable, economically viable and environment friendly fermentation system. Biofilm of Bacillus subtilis was investigated for repeated batch fermentation for the production of protease enzyme. It was found that complete replacement of spent medium with fresh medium supplementation in biofilm produced similar amount of protease that is about 225 U/ml of enzyme in successive batches. Moreover, unlike some solid carriers the EPS layer does not entrap much enzyme inside it. Therefore, instead of using immobilized cells, biofilm of B. subtilis can be a good candidate for protease enzyme production using repeated batch fermentation. Jahangirnagar University J. Biol. Sci. 6(2): 29-38, 2017 (December)