Healing Rate of Ruminal Incision in Sheep After Single Dose Effect of Low Level Laser Therapy

To assess the effect of single dose of low level laser therapy on ruminal healing after rumenotomy, twelve male Awassi local sheep weighing (30 ± 5) Kg and aging (10 ± 2) months, the animals were divided randomly into two groups, (treatment and control groups) each group include six rams equally, collection of venous blood for total white blood cells counts (WBCs) before surgery, 24 hours after surgery, one week and two weeks post operation, all animal subjected to a routine procedure of rumenotomy and after suturing the rumen; the treatment group exposed to a single dose of low-level laser therapy has 820 nm, 1000 mw, 10 sec, 16 J/cmsq, 146 Hz pulsing rate per second while the control group had to suture only. The total count of WBCs showed there were elevations in the treatment group when compared with control group in 24hrs after surgery, a week post operation and two weeks post operation. The histopathological examinations revealed that the healing processes in the treatment group were faster and better exhibited the granulation tissue characterized by vertical formation of blood vessels with the site of incision and illuminated that the newly blood vessels contained RBCs and proliferation of fibroblasts enriched with newly formed blood vessels when compared with the control group which appeared with low in rate and type of healing process after ten days post-surgery. We conclude that Single dose of LLLT is considered as a fast, cheap, and effective technique which could increase healing processes and/or defense mechanisms after rumenotomy in sheep