Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide

A new, fast, accurate, reliable and stability indicating HPLC method for the determination of Diflurobenzuron (DFB), in technical and formulation samples in presence of related and degraded impurities has been developed using intersil – 3 stainless steel C. 18 column (5 μm, 250 mm length x 4.6 mm id), acetonitrite: water: 1.4 dioxane (58:39:0.03 v/v) as mobile phase and diphenyl as internal standard. The flow rate of the mobile phase was 2 cm 3 min -1. Detection was carried out at 260 mm using UV detector. The retention times were 1.55 min, 1.75 min, 6.3 min, 7.9 min and 10.7 min for 2, 6 diflurobenzoic acid, 2, 6 diflurobuzamide, diflurobenzuron, difur (an impurity) and diphenyl (internal standard) respectively. The linearity range of DFB was 0.5 to 15 mg per 100 cm 3. The LOD and LOQ values for DFB were 0.142 to 0.432 respectively. When various technical and formulated smples were analysed by this proposed method the percentage recoveries were found to be 99.20-101.80% with RSD between 0.01% to 0.45%.