Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope

The problem of bad contrast in conventional microscopy is well-known and was solved in part by colouring the samples. It is shown theoretically that a laser Fourier holographic microscope produces images undisturbed by speckle-noise. A laser holographic microscope (LHM) is investigated experimentally. The instrument uses visible radiation of λ = 0.514 μm, Mach – Zehnder scheme optical setup, and CCD detector of the hologram. Images are reconstructed digitally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied without any drying as for electron microscope. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-quality Nikon conventional optical microscope with immersion oil and green filter indicates dramatically different contrast. The ultrahigh contrast of the LHM gives much more micromorphological information.