Study on the Effect of Several Natural Products on Tyrosine Damage Induced by Peroxynitrite

Peroxynitrite (ONOO ˉ), a powerful oxidant, is produced by nitric oxide (NO.) and superoxide anion (O 2. ˉ). Under the physiological condition, the ONOO ˉ could oxidize the lipids, nitrifyproteins, damage DNA and others biomolecules, thereby harm human health. The study used natural products Capsanthin, Myricetin and Capsaicin as the research object and controlled with Vc, and developed the method of HPLC-DAD to separate the components of nitrification damage system, which could determine the inhibition rate of natural products on the formation of 3-nitrotyrosine (3-NT). The fluorescence spectrometry was employed to determine the ability of these substances to inhibit tyrosine dimer and inhibit the self-oxidation of phthalate. The results showed that Capsanthin, Myricetin and Capsaicin had strong inhibitory effect on the generation capacity of 3-NT and tyrosine dimer, and strong inhibitory effect on the self-oxidation of phthalate.