Study on Preparation of Immobilized Alkaline Protease and Its Performance

The nano-Fe 3O 4 particles were prepared by hydrothermal method. Cellulose-based magnetic Fe 3O 4 was prepared by adding cellulose solution dissolved in sodium hydroxide / thiourea / urea system. The basic protease was used as the catalyst and glutaraldehyde was used as the crosslinking agent, The production of magnetic recyclable immobilized enzyme can greatly reduce the cost of hydrolysis of sludge. The aldehyde groups in the glutaraldehyde and the amino groups in the protein can be stably crosslinked by coupling. The immobilization effect of nanometer Fe 3O 4 was reflected by investigating the activity of immobilized enzyme and immobilization rate of enzyme under different conditions.