Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors

Like all cancers, Gastric cancer (GC) is also known to be of genetic origin. Genes are mutated in every steps of cell division, among them hMLH1 is a DNA mismatch gene which plays important role during carcinogenesis. This study was chalked out to find out the status of mutated hMLH1 Gene and its clinicopathological relevance among Bangladeshi gastric cancer patients. It was a cross sectional study conducted during January 2015 to April 2016. Tissue extracted from gastrectomy specimen of carcinoma stomach patients sent to specified laboratory. In the laboratory after DNA extraction, PCR, sequencing and analysis was done. In this study out of 19, exons 7 and 8 and introns 8 and 9 were studied after primer designing for hMLH1 gene mutation. After analysis, mutated gene were matched with clinicopathological parameters like age, sex, location of tumour, types and grade of tumour to see their relevance. Out of 45 patients, mutation was found in 15 patients (33.3%). Most gene alteration was found in elderly patients, more in male. Antral growth had more (80.0%) mutation and in ulceroproliferative type (66.7%) (p < .005). Mutation was common in intestinal type (73.3%) of GC. hMLH1 gene mutation found more in the moderately differentiated carcinoma patients. Factors like age, sex, morphology, Lauren’s type, grading, personal habits etc. might play pivotal role in the development of gene mutation. Multi-centre large study are required to extract more relevant information in this regard