Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells

In cancer patients, oxidative stress (OS) plays an important role in the initiation, promotion, progression of disease, in addition to, the continuous exposure to OS throughout the surgical procedure, chemotherapy and radiotherapy. The present study was designated to evaluate the role of vitamin D3 and B6 supplementation in combination with anti-cancer therapies in maintenance the redox status and their impact on angiogenesis, the main step in cancer progression and aggressiveness, as well as on the side effect of surgery, chemotherapy and/or radiotherapy. Our investigations were at two levels, firstly, assessment of chemical parameters for redox status and angiogenic factors, secondly, assessment level of circulating endothelial cells by applying immunoassay using antibodies coupling nanoparticles. Blood samples were collected once from 15 apparently healthy women (GP-I) and four times from 20 BC patients; (GpII)before mastectomy (B. M.), after mastectomy (A. M.), after ending of chemotherapy course (A. C.) and after 3 months later (A.3M) for both subgroups to Monitor the oxidant-antioxidants status (MDA and TAS) and angiogenic biomarkers (VEGF, ES and CECs). Our data reveals that comparing to control group, BC patients show significant elevation of MDA, VEGF and CECs (P<0.001) as well as ES (P<0.05). The serum level of CECs decreased significantly (P<0.001) in BC patients supplemented with vitamins from the beginning of chemotherapy comparing to those receiving chemotherapy alone and then supplemented with vitamins for three months later. Supplementation with vitamins D3 and B6 reduces the oxidative stress, the side effects of chemotherapy and radiotherapy as vomiting and fatigue, in addition to interfere with angiogenesis