Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon

Miniaturized galleries are essential for a good identification of bacteria. The aim of this study was to identify multi-resistant Klebsiellap neumoniae strains in the referral health structures of Cameroon littoral region and establish a digital profile. A cross-sectional and descriptivestudy was conducted during 2016 and early 2017, intermediate susceptibility isolates or resistant to cephalosporins third generation (C3G) were collected in the laboratories of referral hospitals of the Littoral (General Hospital, Laquintinie, Military, Gyneco-obstetrics and pediatrics of Douala and Saint John of Malta of Njombé) and in Diagmed laboratory. The confirmation of the strains was made by the gallery API 20E？ (BioMérieux). In this study, atotal of 412 strains of Klebsiellasp (species) were isolated. We obtained 125 strains of Klebsiellasp intermediate or resistant to (C3G) and 122 were confirmed to be Klebsiella pneumoniae. The sex ratio (F / M) was 1.26. Klebsiella pneumoniae pneumonia was the species mostly identified at 82.4% and the two digital profiles mainly found were 5215773 and 1215773 with respective frequencies of 52.8% and 18.4%. The biochemical profile: LDC (+), CIT (+), URE (+), IND (-), VP (+), GLU (+), MAN (+), INO (+), SOR (+), RHA (+), SAC (+), MEL (+), ARA (+) was found in all services. There is a high frequency of Klebsiella pneumoniae pneumonia with several different digital profiles, so it is still essential to make a good identification to facilitate the epidemiological data