Dried leaves of Eucalyptuscamaldulensis were finely grinded and fractionated by sieving into four granulometric classes (<100 μm, 100 - 200 μm,
200 - 355 μm and >355 μm). The obtained powder fractions were used for
essential oil (EO) extraction by hydrodistillation and their phytochemical
profile and invitro antioxidant activities were evaluated. The mother powder (unsieved powder) was
used for comparison. Particle size exerted a significant influence (p < 0.05) on the phytochemical composition and invitro antioxidant properties of
the EOs. Comparatively, the mother powder had the highest contents of α-pinene (55.6%), camphene (3.4%) and limonene
(3.7%), while 1,8-cineole (26.6% and 22.4%), exo-fenchol (5.6% and
3.5%), α-campholenol (4.2% and 3.4%),
L-trans-pinocarveol (5.5% and 2.7%), L-borneol (12.6% and 6.8%) and α-terpineol (16.4% and 7.6%) are the
main compounds of EOs from the <100 μm and 100 - 200 μm fractions,
respectively. The antioxidant activities of the EOs revealed higher
radical-scavenging activities DPPH (90.62% and 70.46%) and ABTS (89.59% and
73.31%) for finer fractions (<100 μm and 100 - 200 μm, respectively). The
best reducing power (36.15% and 34.27%) were also found in these finer powder
fractions which improved by more than 2 times the value of mother powder
(reducing power of 17.01%). These results suggest that grinding followed by
sieve fractionation concentrates the majority of antioxidant phytochemicals in
the EOs of the finer powder fractions of E.
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