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OSMAC策略在费希新萨托菌次级代谢产物研究中的应用
Study on the Secondary Metabolites of Neosartorya fischeri via the OSMAC Method

DOI: 10.12677/AMB.2021.101003, PP. 14-20

Keywords: OSMAC，次级代谢产物，分离，费希新萨托菌
OSMAC, Secondary Metabolites, Separation, Neosartorya fischeri

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Abstract:

本研究选取根据基因预测结果存在大量生物合成基因簇但目前次级代谢产物研究的仍较少的费希新萨托菌(Neosartorya fischeri NRRL 181)作为研究对象，先利用OSMAC策略研究了不同培养基下费希新萨托菌的次级代谢产物情况，再选择了大米作为培养基对该菌进行了大规模发酵。发酵结束后对95%乙醇提取所得的浸膏利用传统的柱层析手段进行了系统的次级代谢产物分离工作，共分离并鉴定了8个化合物。
It seems that Neosartorya fischeri NRRL 181 has a large number of biosynthetic gene clusters based on the predicting results of gene. But its secondary metabolites are still less studied at present. In this study, the OSMAC strategy was used to study the secondary metabolites of N. fischeri in different media. Then the rice was selected as the medium for large-scale fermentation. After fermentation, the extract obtained from 95% ethanol was used to separate the secondary metabolites using traditional column chromatography. A total of 8 compounds were separated and identified.

References

[1]  何庆, 李青连, 王丽非, 洪斌. 基于微生物基因组的新型天然产物发现策略[J]. 国际药学研究杂志, 2012, 39(1): 1-6+25.
[2]  赵琪, 闵涛玲, 胡海峰. 激活微生物沉默基因簇合成新型天然产物的研究[J]. 世界临床药物, 2014, 35(8): 501-505.
[3]  Gomez-Escribano, J.P. and Bibb, M.J. (2014) Heterologous Expression of Natural Product Biosynthetic Gene Clusters in Streptomyces coelicolor: From Genome Mining to Manipulation of Biosynthetic Pathways. Journal of Industrial Microbiology and Biotechnology, 41, 425-431.
https://doi.org/10.1007/s10295-013-1348-5
[4]  谢绵测, 李先国, 张大海. OSMAC策略及其在烟曲霉菌次级代谢产物研究中的应用[J]. 天然产物研究与开发, 2015, 27(9): 1668-1673.
[5]  卢轩, 陈刚, 裴月湖. 单菌多产物策略及其在药物先导化合物研发中的应用[J]. 沈阳药科大学学报, 2011, 28(8): 640-645.
[6]  韦洪娟, 林贞建, 李德海, 顾谦群, 朱天骄. 单菌多次级代谢产物方法及其在微生物代谢产物研究中的应用[J]. 微生物学报, 2010, 50(6): 701-709.
[7]  刘俊慧. 基于OSMAC策略的海洋微生物次级代谢产物研究[D]: [硕士学位论文]. 大连: 大连理工大学, 2015.
[8]  于桂洪, 李德海, 朱天骄, 顾谦群. 基于OSMAC策略的深海来源真菌Cladosporium sp. 2005-01-E3次级代谢产物研究[C]//中国海洋湖沼学会. “全球变化下的海洋与湖沼生态安全”学术交流会论文摘要集, 2014: 1.
[9]  Wong, S.M., Musza, L.L., Kydd, G.C., Kullnig, R., Gillum, A.M. and Cooper, R. (1993) Fiscalins: New Substance P Inhibitors Produced by the Fungus Neosartorya fischeri. Taxonomy, Fermentation, Structures, and Biological Properties. Journal of Antibiotics, 46, 545-553.
[10]  Morino, T., Nishimoto, M., Itou, N. and Nishikiori, T. (1994) NK372135s, Novel Antifungal Agents Produced by Neosartoria fischeri. Journal of Antibiotics, 47, 1546-1548.
[11]  Wakana, D., Hosoe, T., Itabashi, T., Nozawa, K., Okada, K., Takaki, G.M.D., Yaguchi, T., Fukushima, K. and Kawai, K. (2006) Isolation of Isoterrein from Neosartorya fischeri. Mycotoxins, 56, 3-5.
[12]  Tan, Q.W., Ouyang, M.A., Shen, S. and Li, W. (2012) Bioactive Metabolites from a Marine-Derived Strain of the Fungus Neosartorya fischeri. Natural Product Research, 26, 1402-1407.
https://doi.org/10.1080/14786419.2011.592834
[13]  Eamvijarn, A., Gomes, N.M., Dethoup, T., Buaruang, J., Manoch, L., Silva, A., Pedro, M., Marini, I., Roussis, V. and Kijjoa, A. (2013) Bioactive Meroditerpenes and Indole Alkaloids from the Soil Fungus Neosartorya fischeri (KUFC 6344), and the Marine-Derived Fungi Neosartorya laciniosa (KUFC 7896) and Neosartorya tsunodae (KUFC 9213). Tetrahedron, 69, 8583-8591.
https://doi.org/10.1016/j.tet.2013.07.078
[14]  Zheng, Z.Z., Shan, W.G., Wang, S.L., Ying, Y.M., Ma, L.F. and Zhan, Z.J. (2014) Three New Prenylated Diketopiperazines from Neosartorya fischeri. Helvetica Chimica Acta, 97, 1020-1026.
https://doi.org/10.1002/hlca.201300416
[15]  Chen, B.Y., Wang, Z., Ying, Y.M., Jiang, L.X., Zhan, Z.J., Wang, J.L. and Zhang, W. (2014) Neofipiperzine D, a New Prenylated Indole Alkaloid Metabolite of the Fungus Neosartorya fischeri. Journal of Chemical Research, 38, 539-541.
https://doi.org/10.3184%2F174751914X14097355956467
[16]  Yin, W.B., Grundmann, A., Cheng, J. and Li, S.M. (2009) Acetylaszonalenin Biosynthesis in Neosartorya fischeri. Identification of the Biosynthetic Gene Cluster by Genomic Mining and Functional Proof of the Genes by Biochemical Investigation. Journal of Biological Chemistry, 284, 100-109.
https://doi.org/10.1074/jbc.M807606200
[17]  Kang, J., Wang, H.-Q. and Chen, R.-Y. (2003) Studies on the Constituents of the Mycelia Produced from Fermented Culture of Flammulina velutipes. International Journal of Medicinal Mushrooms, 5, 391-396.
[18]  Huang, H.C., Liaw, C.C., Yang, H.L., Hseu, Y.C., Kuo, H.T., Tsai, Y.C., Chien, S.C., Amagaya, S., Chen, Y.C. and Kuo, Y.H. (2012) Lanostane Triterpenoids and Sterols from Antrodia camphorate. Phytochem, 84, 177-183.
https://doi.org/10.1016/j.phytochem.2012.08.011
[19]  唐京生, 陈谨, 田军, 王幼平, 吴凤锷. 峨眉海桐化学成分的研究[J]. 四川大学学报, 2002, 39(3): 538-541.
[20]  马伟光, 李兴从, 王德祖. 松橄榄中的麦角甾醇类过氧化物[J]. 云南植物研究, 1994, 16(2): 196-200.
[21]  Ciminiello, P., Fattorusso, E., Magno, S., Mangoni, A. and Pansinit, M. (1990) Incisterols, a New Class of Highly Degraded Sterols from the Marine Sponge Dictyonella Incise. Journal of the American Chemical Society, 112, 3505-3509.
https://doi.org/10.1021/ja00165a039
[22]  Kawahara, N., Sekita, S. and Satake, M. (1994) Steroids from Calvatia cyathiformis. Phytochemistry, 37, 213-215.
https://doi.org/10.1016/0031-9422(94)85028-3

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