Introduction: Skin aging is the result of many cellular dysfunctions over time
particularly the fibroblasts and the keratinocytes. These dysfunctions could be
decelerated by the preventive effects of some skin treatments such as Intradermal
injections. NCTF 135HA has a polycomponent formulation designed to improve the efficacy of
non-cross linked hyaluronic acid (as a micro-filler) on fibroblasts function.
Although NCTF 135HA has been used by aesthetic practitioners since 20 years, we
have analyzed all in vitro, ex vivo and in vivo studies during past 10 years in order to summarized its anti-aging effect. Methods: In these series of studies, the known effects of HA have been evaluated with this
boosted formula. Collagen I synthesis, antiglycation effect and contractile
forces developed by fibroblasts were studied. Fibroblasts and keratinocytes
proliferations were evaluated in monolayer cells culture. The filling effect
thanks to NCTF 135HA injection was ex
vivo performed by fringe projection and the protective effect of NCTF 135HA
against solar irradiation was performed ex
vivo to study proliferation rate, elastin and collagen expression. Two
clinical trials were performed on women to evaluate the anti-aging effect of
NCTF 135HA injection. Hydration, firmness, radiance, wrinkles size, pore size,
thickness and density of the dermis were analyzed. Results: Collagen I, anti-AGE products, cell proliferation and contractile forces
were significantly increased with NCTF 135HA in culture medium. Ex vivo studies showed that NCTF 135HA
increased, cells proliferation rate, elastin and collagen production. In
addition, the filling effect of the NCTF 135HA is significantly superior to
placebo. The first clinical trial shows that NCTF 135HA injection significantly
increased tonicity, hydration and radiance of the skin and significantly
decreased wrinkle score. The second clinical trial demonstrated that NCTF 135HA
injection significantly increased dermis density and thickness, skin
homogeneity and skin radiance and significantly decreased pore size and wrinkle
depth and volume. Conclusion: In vitro, ex vivo and in vivo studies showed that this minimally invasive technique could be used in order to
improve the skin quality which could have a visual effect on preventing the
aging signs.
References
[1]
Farage, M.A., Miller, K.W., Elsner, P. and Maibach, H.I. (2013) Characteristics of the Aging Skin. Advances in Wound Care (New Rochelle), 2, 5-10. https://doi.org/10.1089/wound.2011.0356
[2]
Waller, J.M. and Maibach, H.I. (2005) Age and Skin Structure and Function, a Quantitative Approach (I): Blood Flow, pH, Thickness, and Ultrasound Echogenicity. Skin Research and Technology, 11, 221-235. https://doi.org/10.1111/j.0909-725X.2005.00151.x
[3]
Boss, G.R. and Seegmiller, J.E. (1981) Age-Related Physiological Changes and Their Clinical Significance. The Western Journal of Medicine, 135, 434-440.
[4]
McCallion, R. and Li Wan Po, A. (1993) Dry and Photo-Aged Skin: Manifestations and Management. Journal of Clinical Pharmacy and Therapeutics, 18, 15-32. https://doi.org/10.1111/j.1365-2710.1993.tb00562.x
[5]
Südel, K.M., Venzke K., Mielke, H., Breitenbach, U., Mundt, C., Jaspers, S., Koop, U., Sauermann, K., Knussman-Hartig, E., Moll, I., Gercken, G., Young, A.R., Stäb, F., Wenck, H. and Gallinat, S. (2005) Novel Aspects of Intrinsic and Extrinsic Aging of Human Skin: Beneficial Effects of Soy Extract. Photochemistry and Photobiology, 81, 581-587. https://doi.org/10.1562/2004-06-16-RA-202.1
[6]
Duncan, K.O. and Leffell, D.J. (1997) Preoperative Assessment of the Elderly Patient. Dermatolic Clinics, 15, 583-593. https://doi.org/10.1016/S0733-8635(05)70468-X
[7]
Haneke (2006) Skin Rejuvenation without a Scalpel. I. Fillers. Journal of Cosmetic Dermatology, 5, 157-167. https://doi.org/10.1111/j.1473-2165.2006.00243.x
[8]
Savoia, A., Landi, S. and Baldi, A. (2013) A New Minimally Invasive Mesotherapy Technique for Facial Rejuvenation. Dermatology and Therapy, 8, 83-93. https://doi.org/10.1007/s13555-012-0018-2
[9]
Lorizzo, M., De Padova, M.P. and Tosti, A. (2008) Biorejuvenation: Theory and Practice. Clinics in Dermatology, 26, 177-181. https://doi.org/10.1016/j.clindermatol.2007.09.011
[10]
Prikhnenko, S. (2015) Polycomponent Mesotherapy Formulations for the Treatment of Skin Aging and Improvement of Skin Quality. Clinical, Cosmetic and Investigational Dermatology, 8, 151-157. https://doi.org/10.2147/CCID.S76721
[11]
Zhao, J.Y., Chai, J.K., Song, H.F. and Xu, M.H. (2012) Experimental Study of Hyaluronic Acid on the Biomechanical Compliance of Porcine Acellular Dermal Transplantation. The National Medical Journal of China, 92, 1428-1430.
[12]
Bell, E., Ivarsson, B. and Merril, C. (1979) Production of a Tissue-Like Structure by Contraction of Collagen Lattices by Human Fibroblasts of Different Proliferative Potential in Vitro. Proceedings of the National Academy of Sciences of the United States of America, 3, 1274-1278. https://doi.org/10.1073/pnas.76.3.1274
[13]
Armbruster, V., Gharbi, T., Viennet, C. and Humbert, Ph. (2004) Silicon Grid Devices for Attachment of Cultured Fibroblast Collagen Lattices. Sensor and Actuators A: Physical, 116, 219-223. https://doi.org/10.1016/j.sna.2004.03.065
[14]
Robin, S., Courderot-Masuyer, C., Tauzin, H., Guillon, S, Gaborit, J., Harbon, S. and Humbert, Ph. (2012) Evaluation of Protective and Restoring Effects of a Mixture of Silanols on Photoaging. Use of a Device Allowing the Quantification of Contractile Strengths of Human Fibroblasts after UVA Irradiation. International Journal of Cosmetic Science, 34, 311-317. https://doi.org/10.1111/j.1468-2494.2012.00716.x
[15]
Humbert, Ph., Fanian, F., Lihoreau, T., Jeudy, A., Elkhyat, A., Robin, S., Courderot-Masuyer, C., Tauzin, H., Lafforgue, C. and Haftek, M. (2015) Mecano-Stimulation of the Skin Improves Sagging Score and Induces Beneficial Functional Modification of the Fibroblasts: Clinical, Biological and Histological Evaluations. Clinical Interventions in Aging, 10, 387-403. https://doi.org/10.2147/CIA.S69752
[16]
Courderot-Masuyer, C., Robin, S., Tauzin, H. and Humbert, Ph. (2016) Evaluation of Lifting and Antiwrinkle Effects of Calcium Hydroxylapatite Filler. In Vitro Quantification of Contractile Forces of Human Wrinkle and Normal Aged Fibroblasts Treated with Calcium Hydroxylapatite. Journal of Cosmetic Dermatology, 15, 260-268. https://doi.org/10.1111/jocd.12215
[17]
Varani, J. (2010) Fibroblast Aging: Intrinsic and Extrinsic Factors. Drug Discovery Today: Therapeutic Strategies, 7, 65-70. https://doi.org/10.1016/j.ddstr.2011.06.001
[18]
Jäger, C., Brenner, C., Habicht, J. and Wallich, R. (2012) Bioactive Reagents Used in Mesotherapy for Skin Rejuvenation in Vivo Induce Diverse Physiological Processes in Human Skin Fibroblasts in Vitro—A Pilot Study. Experimental Dermatology, 21, 72-75. https://doi.org/10.1111/j.1600-0625.2011.01400.x
[19]
Wang, F., Garza, L.A., Kang, S., Varani, J., Orringer, J.S., Fisher, G.J. and Voorhees, J.J. (2007) In Vivo Stimulation of de Novo Collagen Production Caused by Cross-Linked Hyaluronic Acid Dermal Filler Injections in Photodamaged Human Skin. Archives of Dermatology, 143, 155-163. https://doi.org/10.1001/archderm.143.2.155
[20]
Weindl, G., Schaller, M., Schäfer-Korting, M. and Korting, H.C. (2004) Hyaluronic Acid in the Treatment and Prevention of Skin Diseases: Molecular Biological, Pharmaceutical and Clinical Aspects. Skin Pharmacology and Physiology, 17, 207-213. https://doi.org/10.1159/000080213
[21]
Courderot-Masuyer, C. (2017) Mechanical Properties of Fibroblasts. In: Humbert, P., Fanian, F., Maibach, H. and Agache, P., Eds., Agache’s Measuring the Skin, Springer, Cham, 903-909. https://doi.org/10.1007/978-3-319-32383-1_125
[22]
Vedrenne, N., Coulomb, B., Danigo, A., Bonté, F. and Desmoulière, A. (2012) The Complex Dialogue Between (Myo)fibroblasts and the Extracellular Matrix during Skin Repair Processes and Ageing. Pathologie Biologie (Paris), 60, 20-27. https://doi.org/10.1016/j.patbio.2011.10.002
