流动注射化学发光法测定邻氨基酚含量的研究
The Study Determination of o-Aminophenol with Flow Injection Chemiluminescence Method

在酸性介质中,Fe3+-过氧化氢体系生成羟基自由基,强氧化活性的羟基自由基与邻氨基酚产生化学发光反应,罗丹明6G能够增敏化学发光信号,为测定邻氨基酚的含量,建立了灵敏的流动注射化学发光分析法.研究了影响化学发光的各种因素,探讨了可能的机理.邻氨基酚浓度在1.0×10-4～1.0×10-2、1.0× 10-2～7.0× 10-1和7.0× 10-1～3.0× 100 g/L范围内与化学发光信号强度值呈良好的线性关系,检测限为(30)3.0× 10-5 g/L.对1.0×10-3g/L的没食子酸溶液进行11次平行测定,相对标准偏差为3.9；.应用本方法测定模拟样品中邻氨基酚的含量,进行了加标回收实验,结果满意.
In this paper, a sensitive method for the determination of o-aminophenol was developed. It was based on the enhanceed chemiluminescent emitted during the oxidation of o-aminophenol by produce of strong oxidation active hydroxy free radical with chemical reaction between Fe3+ and H2O2 solution in acid medium in the presence of rhodmine 6G as energy transfer in chemiluminescence system. Various factors influenced the CL system were investigated carefully, the possible mechanism of the proposed method was also studied preliminarily. The detection limit is 3×10?5 g/L(3σ), the linear range of determination was 1.0×10?4~1×10?2, 1.0×10?2~7.0×10?1 and 7.0×10?1~ 3.0×100 g/L, the relative standard deviation of 3.9% (1.0×10?3 g/L, n=11). The recommended method has been successfully used for analysis of o-aminophenol in simulation samples