E型莰烯醛肟、莰烯腈的合成与抑菌活性
The Synthesis and Antibacterial Activity of E-Type Camphene Aldoxime and E-Type Camphene Nitrile

将E型莰烯醛与盐酸羟胺在碳酸钠作用下反应合成了E型莰烯醛肟,将E型莰烯醛肟用乙酸酐脱水得到E型莰烯腈,产品得率和纯化均在90%以上.产物结构均经MS和NMR分析方法进行了表征.采用菌丝生长速率法,将E型莰烯醛肟和莰烯腈对12种植物病原真菌的生长进行了抑制活性试验,实验结果表明:在药液质量浓度为500 mg?L-1时,E型莰烯醛肟对油茶炭疽病菌(GlomerellaCingulata,A)、玉米赤霉病菌(Gibberellazeae,B)、梨链格孢菌(Alternariakikuchiana,C)、辣椒菌核病菌(Sclerotiniasclerotiorum,D)、水稻纹枯病菌(Thanatephoruscucumeris,E)、辣椒疫霉病菌(Phytophthoracapsici,F)、毛竹枯梢病菌(Ceratosphaeriaphyllostachydis,G)、猕猴桃果实拟茎点霉(Botryisphariadothide,H)、葡萄炭疽病菌(Colletorichumgloeosporioides,K)的抑制率均达100%; E型莰烯腈对所试植物病原真菌的抑制率均高于98.5%,多数为100%.当药液质量浓度为250 mg?L-1时,E型莰烯醛肟对A、B、E、F、G、H、K等7种植物病原真菌的抑制率仍达95%以上.抑制效果超过甚至远超过百菌清的抑制效果.
E-type camphene aldehyde oxime is synthesized by the reaction of E-type camphene aldehyde with hydroxylamine hydrochloride under the action of sodium carbonate.E-type camphene aldehyde oxime is dehydrated with acetic anhydride to obtain E-type camphene nitrile.The yield and purity of the product are over 90%.The structures of the products are characterized by MS and NMR.The inhibitory activity of E-type camphene aldoxime and camphene nitrile on the growth of 12 plant pathogenic fungi is tested by mycelial growth rate method.The results show that under the concentration of 500 mg?L-1,the inhibition rate of E-camphene aldoxime on GlomerellaCingulata(A),Gibberellazeae(B),Alternariakikuchiana(C),Sclerotiniasclerotiorum(D)and Thanatephoruscucumeris(E),Phytophthoracapsici(F),Ceratosphaeriaphyllostachydis(G),Botryisphariadothide(H),Colletotrichumgloeosporioides(K)all reach 100%.The inhibition rate of E-type camphene nitrile on plant pathogenic fungi is higher than 98.5%,most of which are 100%.When the concentration of the solution is 250 mg?L-1,the inhibition rate of E-camphene aldoxime to 7 plant pathogenic fungi,such as A,B,E,F,G,H,K is still more than 95%.The inhibition rate of 2 compounds exceeds or even far exceeds that of chlorothalonil