医用空气过滤装置对粉尘螨杀灭的实验研究
The Experimental Study on Acaricidal Activity of Medical Air Purification Device Against Dermatophagoides farinae

为探讨医用空气过滤装置对粉尘螨杀灭效果及降低空气中尘螨过敏原含量的影响,挑取粉尘螨成虫放置在空气过滤装置中,按不同时间段(0,12,24,48,72,96 h)分组,每组200只粉尘螨,处理后采取样本进行杀螨率检测及扫描电镜观察; 采用抗原雾化法检测,收集空气过滤装置处理的1 h样品,并通过双抗体夹心法检测样品中抗原含量.结果表明:空气净化装置处理后尘螨死亡率随着作用时间的延长而逐渐升高,在72 h和96 h,尘螨死亡率分别达到60.5%和92.5%; 处理后的死螨严重脱水,虫体形态高度皱缩.医用空气过滤装置对空气中粉尘螨主要抗原Der f 1和Der f 2具有显著降低作用.
To observe the acaricidal activity of medical air purification device against dust mites and to detect the indoor allergen concentration before and after the air purification device operate,adult dust mites are placed in the air purifier and divided into six groups according to different time points(0,12,24,48,72 and 96 hours),with 200 dust mites in each group.The death of dust mites is counted and observed under scanning electron microscope(SEM).The concentrations of dust mite allergens of which are treated by the air purifier in different timing points were detected by ELISA.The results show that the death rate rise with increasing over time of the air purifier opening.The death rate of dust mites at 72 h and 96 h are respectively 60.5% and 92.5%.Scanning electron microscopy clearly show the dead dust mites showed dry,flat and wrinkled body,curled limbs,significant changes in shapes.The medical air purification device can kill dust mites by creating a relatively dry environment and also reduce the concentrations of dust mite allergens Der f1 and Der f2