牡蛎多肽对长期大负荷训练大鼠血睾酮、LH和StAR mRNA表达的影响
The Effect of Oyster Peptides Supplementation on Serum Testosterone,LH Content and StAR mRNA Expression in Rats During the Long-Term and High Loading Training

为探讨补充牡蛎多肽对长期大负荷训练大鼠血睾酮、LH和StAR mRNA表达的影响.将32只雄性SD大鼠随机分成4组:对照组(QC)、单纯运动组(SE)、运动+低剂量牡蛎多肽组(E+LOP)和运动+高剂量牡蛎多肽组(E+HOP).除QC组外各组每周按照训练方案建立长期大负荷训练模型,E+LOP组和E+ HOP组每次运动结束2h后补充相应剂量的牡蛎多肽,QC组和SE组补充等体积的生理盐水,并且每周末记录各组大鼠质量.5周后麻醉组织取材,取血测定红蛋白(HGB),利用放免法检测各组大鼠血清睾酮(T)和黄体生成素(LH)含量,RT-PCR检测睾丸组织中StAR mRNA表达水平.结果表明:5周后,与QC组比较,SE组HGB、血清T水平在统计学业意义上显著降低(P＜0.05),LH水平无统计学意义上的显著差异(P＞0.05),大鼠质量随着周次增加增重减小(P＜0.01),StAR mRNA表达量低(P＜0.01);与SE组比较,E+ LOP和E+HOP组HGB、血清T水平在统计学业意义上显著升高(P＜0.05),LH水平无统计学意义上的显著差异(P＞0.05),大鼠质量随着周次增加增重变大(P＜0.05),StAR mRNA表达量高(P＜0.05);E+LOP和E+HOP组之间无统计学意义上的显著差异(P＞0.05).因此补充牡蛎多肽可以促进睾丸组织StAR mRNA表达,从而使间质细胞睾酮合成增加,有利于改善长期大负荷训练导致的低血T.
To investigate the effect of oyster peptides supplementation on serum testoserone,LH content and StAR mRNA expression in rats during the long-term and high loading training.Thirty two male rats were randomly divided into four groups:QC,SE,E+LOP and E+HOP.All the groups except QC group followed a protocol of treadmill training,E+LOP and E+HOP groups ingested oyster peptides with the corresponding content after two hours,at the same time,QC and SE consumed physiological saline with the same volume,weight of rats were recorded on the weeken during the training.After 5-week training,all the rats were taken anesthesia for tissue sampling.Contents of HGB,T,and LH were determined,StAR mRNA expressions of the rats'testicular tissue were detected by PT-PCR.Compared with the QC group After five weeks,HGB and serum T Contents of SE group significantly decreased(P<0.05),and StAR mRNA expressions of the rats'testicular tissue significantly decreased,change of the mean body weights became less with the week number increasing(P<0.05); Compared with the SE group,HGB and serum T Contents of E+LOP group and E+HOP group significantly increased(P<0.05),StAR mRNA expressions significantly increased(P<0.05),change of the mean body weights obviously became greater with the week number increasing(P<0.05).but there was no difference in contents of LH among four groups(P>0.05),and there was no difference in the above indicators between E+LOP group and E+HOP group(P>0.05).Oyster peptides supplementation can elevate StAR mRNA expressions of the rats'testicular tissue,and prompt the synthesis of testoserone,which improves symptoms of the low serum testoserone