摘要 目的 研究大麻素Ⅱ型（CB2）受体激活对1-甲基-4-苯基吡啶离子(MPP+)致SH-SY5Y细胞损伤的保护作用。 方法 根据药物处理的不同将细胞分为正常对照组（C组）、MPP+组（M组）、JWH-133/MPP+组（J+M组）以及JWH-133/AM630/MPP+组（J+A+M组）。用免疫印迹法检测各组细胞CB2受体和酪氨酸羟化酶（TH）蛋白的表达，用流式细胞仪检测细胞线粒体膜电位（ΔΨm）的变化。 结果 与C组相比，M组的CB2受体蛋白表达下降（P＜0.01）；经JWH-133预处理后，CB2受体蛋白表达高于M组（P＜0.01），此作用可被AM630所阻断；与C组相比，M组的TH表达降低（P＜0.05），经JWH-133预处理后，TH表达高于M组（P＜0.05），AM630预处理可抑制此作用；与C组相比，M组细胞的ΔΨm明显下降（P＜0.01），经JWH-133预处理后，细胞ΔΨm下降幅度变小（P＜0.01），此作用可被AM630所阻断。 结论 CB2受体激活可以抑制MPP+对SH-SY5Y细胞的损伤作用。 Abstract：Objective To investigate the protective effect of activation of cannabinoid receptor 2 (CB2) against 1-methyl-4-phenylpyridinium (MPP+)-induced injury of SH-SY5Y cells. Methods SH-SY5Y cells were divided into normal control group (group C), MPP+ group (group M), JWH-133/MPP+ group (group J+M), and JWH-133/AM630/MPP+ group (group J+A+M) according to different drug treatment methods. The expression of CB2 and tyrosine hydroxylase (TH) in cells was determined by Western Blotting, and the changes in mitochondrial membrane potential (ΔΨm) were measured by flow cytometry. Results Group M had a significant reduction in the expression of CB2 protein compared with group C (P<0.01). After pretreatment with JWH-133, the expression of CB2 protein was significantly higher than that in group M (P<0.01), but the effect of JWH-133 could be blocked by AM630. Compared with group C, group M had a significant reduction in the expression of TH protein (P<0.05). However, the expression of TH protein was significantly higher than that in group M after pretreatment with JWH-133 (P<0.05), and AM630 pretreatment was found to suppress this effect. Group M had a significant decrease in the ΔΨm of cells compared with group C (P<0.01); the decrease in ΔΨm was reduced after pretreatment with JWH-133 (P<0.01), and the effect of JWH-133 could be blocked by AM630. Conclusion Activation of CB2 can protect SH-SY5Y cells from the damage caused by MPP+
