Study of Bacterial Spectrum in Patients of Chronic Dacryocystitis, At a Tertiary Care Centre in Northern India | OMICS International

Purpose: To ascertain the current pattern of microbial isolates and their antibiotic sensitivity patterns in patients of chronic dacryocystitis seen at a tertiary care centre in North India. Methods: A case record analysis was conducted on computerized data of 400 immune competent patients of chronic dacryocystitis in KGMC, Lucknow between Jan 2011-Dec 2015. The sample for purpose of microbiological isolate was collected from patients who ROPLAS +ve. The sample was collected under strict sterile control and culture was done on 5% sheep blood agar. Antibiotic sensitivity was done by Kirby Bauer disc diffusion method on Mueller Hinton agar. Results: 400 samples were collected for bacterial culture and antibiotic sensitivity. 61% of these samples were sterile at 24 hrs and 72 hrs of culture. 39% were positive for microbial Agents. Staphylococcus aureus (54.6%) was the most common gram positive cocci followed by coagulase negative Staphylococcus epidermidis (19.4%), followed by Streptococcus pneumoniae (14%). Gram negative organisms in both group A and group B included Pseudomonas aeruginosa (6%) followed by Klebsiella pneumoniae (3%) and Hemophilus influenzae (3%). High sensitivity was seen towards vancomycin and fluoroquinolones in Gram positive bacteria and for Piperacillin/ Tazobactum in gram negative cases. Conclusion: It is important to have knowledge of the microbial agent responsible for chronic dacryocystitis in a particular area to the choose the most appropriate antibiotic for the implicated organism