Cytotoxic and cytogenetic effects of aqueous and methanol crude extracts of Nicotiana tabacum on Rhabdomyosarcoma (RD) and L20B cell lines in vitro

The present study was carried out to evaluate the cytotoxicity of aqueous and methanol crude extracts of locally Nicotiana tabacum leaves on two cell lines, Rhabdomyosarcoma (RD) tumor cell line, and Murine fibroblast (L20B) cell line in vitro. The cytogenetic effects of methanol crude extract was studied also on both cell lines by evaluation the mitotic index (M.I) after estimating cytotoxic concentration 50% (CC50%) value for extract. The results of cytotoxic effect of aqueous and methanol crude extracts of N. tabacum revealed that both extracts had a cytotoxic effect against both RD and L20B cell lines, in which a significant decrease in proliferation of RD tumor cell line was observed at 78.125 to 10000μg/ml of methanol crude extracts after 72hrs treatment. Aqueous extract showed cytotoxic effect on both RD and L20B cell lines at all concentrations also after 72hrs treatment. The CC50 values were 2100μg/ml for RD and 2150μg/ml for L20B cell lines after 72hrs exposure with methanol extract. The cytogenetic effects of methanolic crude extract of leaves of N. tabacum revealed a significant decrease in M.I in both cell lines