Identification of Chemical Constituent of Combine Sample of Some Medicinal Plants for Antioxidant Activity

The use of Natural products as medicine has been practiced from ancient time in World. With an estimation of WHO that as many as 80% of world’s population rely on herbal traditional medicines as their primary health care. In recent years the interest to evaluate plants possessing antioxidant activity for various diseases is growing. Based on local use of common diseases and ethno-botanical knowledge, an attempt has been made to investigate the antioxidant activity of combine extract of Aloe barbandesis (leaves), Tinospora cordifolia (stem), Triticum aestivum (straw) Azadirachta indica (leaves) and Ocimum sactum (leaves). In this study the combine extract of selected plant materials, were analysed for their antioxidant activity by peroxide value method and diphenylpicrylhydrazyl radical scavenging method along with the reference sample ascorbic acid. The extract were further analysed to determine their total phenolic content by Folin-Ciocalteau method and total flavonoid content by Dowd method along with the reference sample rutin and gallic acid respectively. The extract exhibited significant antioxidant activity, total phenolic and flavonoid content. The extract can be used effectively for medication purposes