In vitro propagation of Kalanchoe blossfeldiana using BA and NAA

Micropropagation is an efficient in vitro propagation method for Kalanchoe blossfeldiana. In the present research, the effect of plant growth regulators (PGRs) on root formation and adventitious shoot from apical buds explants of Kalanchoe blossfeldiana. Apical buds as explants were cultured on solid MS medium supplemented with BA and NAA, both with concentrations of 0, 0.5, 1 and 2 mg l-1. The experimental design was R.C.B.D. MS medium supplemented with 1 mg l-1 BA + 1 mg l-1 NAA resulted in the highest plant height (6.22 cm), root number (9.34) and root length (10.36 cm). Maximum shoot number (5.39) was obtained in MS medium containing 1 mg l-1 BA + 0.5 mg l-1 NAA. About 85% of the micropropagated plantlets were established successfully in acclimatization medium. Regenerated plantlets were morphologically identical with mother plants.