In vitro Shoot Regeneration of Bacopa monnieri (L.) Using Cyanobacterial Media- A Novel Approach and Effect of Phytoregulators on in vitro micropropagation

This review highlights the recent development and novel achievements made for the multiple shoots regeneration of Bacopa monnieri in Cyanobacterial medium as well as in MS medium. Shootlets were regenerated from nodal explants of stem through auxiliary shoot proliferation. In Cyanobacterial culture medium Bacopa monnieri survived for long period of time and multiple shoot initiation was observed in 2mg/l Kn. When Bacopa monnieri was inoculated in MS medium supplemented with different concentrations of PGR’s, maximum no. of shoots were observed in 0.5 mg/l BAP + 2.0 mg/l Kn and 0.5 mg/l Kn+1.0 mg/l BAP