Ex situ bioremediation of soil contaminated with crude oil by use of actinomycetes consortia for process bioaugmentation

The potential effects of sawdust, and mixture of cow and sheep dung to biostimulant autochthonous microflora and augmentation for hydrocarbon bioremediation were investigated in test biopile, made of soil polluted with petroleum waste 100kg. The soil was fluffed by 1.5% sawdust, then supplemented with the necessary minerals and watered to provide conditions favoring microorganism growth. industrial aeration was provided in pile by drainage-pip network to simulate bioremediation treatments through a 90- day period. During this period, we monitored total petroleum hydrocarbons and changes in bacterial communities. The (TPHs) had been reduced from 52 to 10.6 g.kg-1. In soil, the dominant microorganism population comprised Gram-positive bacteria from actinomycete group and autochthonous microorganisms which decompose hydrocarbons reached highest level 1.6 x 107 cfu.g-1 at 45 days. Based on these data, we conclude that is ex situ (Biopile) experiment the best strategy, inexpensive, efficient, and environmentally friendly and may thus offer a viable choice for petroleum hydrocarbonscontaminated soil remediation