Antifungal activity of Plumbagin and Isodiospyrin from Diospyros kaki root bark

The chloroform extract of root bark of D. kaki exhibited remarkably good antifungal activity at against all the fungi. The bioassay guided fractionation of the crude extract was the done using benzene-chloroform step gradient by silica gel column chromatography. The active compounds A & B obtained were analysed by EI-MS, FTIR and 1H and 13C NMR spectroscopy. On comparision of their spectra with literature they were identified as napthoquinones, Plumbagin (5-hydroxyl, 2-methyl 1,4 -Napthoquinone) and Isodiospyrin (5-hydroxy-6-(1-hydroxy-6-methyl-5,8- dioxo-naphthalen-2-yl)-2-methyl-naphthalene-1,4-dione). The MIC values of the crude extract and compounds A & B were (160-2500 μg/ml), (1.25-20 μg/ml) and (5-160 μg/ml) & the MFC values were (630-5000), (5-160 μg/ml) and (40-160 μg/ml) respectively