Evaluation of In vitro Anti-inflammatory Activity of Viburnum punctatum Buch-Ham. Ex D.Don

The present study was to estimate the preliminary phytochemical screening and in vitro anti-oxidant activity of aerial part extracts of Viburnum punctatum by using solvents like Petroleum ether, Chloroform, Methanol and Water. Preliminary phytochemical analysis reveals the presence of alkaloids, glycosides, flavonoids, phenolic compounds, proteins, phytosterols and saponins. The chloroform and methanol extract were screened for its anti-inflammatory activity by cyclooxygenase inhibition and lipooxygenase inhibition on Human peripheral lymphocytes cell lines using Aspirin as a standard drug. The chloroform and methanol extracts of the Viburnum punctatum was taken at three different concentrations (100μg/ml, 500μg/ml, and 1000μg/ml) and its percentage inhibition was compared with that of the standard Aspirin. The methanolic extract showed good results with that of the standard and hence proved to have Cyclooxygenase and lipo-oxygenase inhibitory activity higher than that of the chloroform extract