In-vitro callus induction from two different explants stem and leaf in Carthamus- tinctorius Linn.

In present study, callus was raised from two different explants stem and leaf of Carthamustinctorius. The explants were cultured from the 3 week old seedlings and transferred to an MS medium supplemented with different concentration of Auxin, 2, 4-D growth regulator hormones.The highest percentage of callus induction (90%) was observed in all surfaces of leaf explants.The medium supplemented with 2, 4-D was more effective that IAA for callus formation