小立碗藓-灰霉菌互作过程中活性氧代谢及病程相关蛋白1转录表达的变化
The Changes on Metabolism of Reactive Oxygen Species and Expression of Pathogenesis Related Protein 1 During Interaction of Physcomitrella patens with Botrytis cinerea

为了解苔藓植物小立碗藓与病原真菌灰霉菌的互作关系,观察了灰霉菌在小立碗藓配子体中的感染行为和小立碗藓受感染后的组织病理学变化.另外,还测定了接种灰霉菌后小立碗藓配子体的过氧化氢量、过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的活性以及病程相关蛋白1(PR1)基因转录表达变化.结果表明:(i)在灰霉菌接种早期小立碗藓出现类似维管束植物的过敏性反应；(ii)接种早期PR1基因表达上调和POD活性增高,说明PR1和POD可能与小立碗藓对灰霉菌的早期防御反应有关；(iii)病原菌侵染引起小立碗藓配子体内CAT、SOD活性下降和过氧化氢量升高,说明小立碗藓与灰霉菌互作中出现了氧化还原态的变化.
To understand histopathology changes during interaction of Physcomitrella patens with Botrytis cinerea,the infection behavior of B.cinerea and the lesion on P.patens were observed by microscopes.In addition,H2O2 generation was detected,transcription expression of PR1 gene and activity of peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)in P.Patens after inoculation with B.cinerea by biochemical and molecular biological mothers.The results showed that:(i)a disease resistance response which is similar to hypersensitive response in vascular plants occurred in P.patens during early stages of the pathogen infection;(ii)the ehanced PR1 gene expression of P.patens and promoted POD activity of P.patens after inoculation with B.cinerea,suggested that PR1 and POD might involved in defense response of bryophyte against B.cinerea.(iii)the increase of H2O2 generation and decrease of CAT and SOD activity caused by B.cinerea infection,suggested that P.patens occurred changes of redox status after inoculation with B.cinerea