摘要 目的 探讨非肌层浸润性膀胱癌(NMIBC)与肌层浸润性膀胱癌(MIBC)谷氨酰胺(Gln)代谢依赖性的差异。 方法 利用Oncomine数据库检索相关NMIBC与MIBC谷氨酰胺酶(Gls)差异性表达的文献并进行分析。通过免疫组化技术分别检测NMIBC和MIBC病理组织中Gls的表达水平。利用MTT、免疫印迹、细胞凋亡等实验方法分析比较Gln依赖性不同的膀胱癌细胞系T24、5637在Gln存在或缺乏条件下的Gls蛋白表达、细胞增殖以及凋亡水平。 结果 与NMIBC相比，MIBC组织中Gls表达水平更高。Gln依赖程度不同的肿瘤细胞T24和5637的Gls表达水平不同。针对Gln依赖性肿瘤细胞T24，Gln的浓度越高，其Gls的表达水平越高，细胞增殖越快，凋亡越少。 结论 NMIBC和MIBC存在Gln代谢依赖性差异，Gln缺乏可能通过促进肿瘤细胞凋亡而影响MIBC的存活。 Abstract：Objective To investigate the difference in dependence on glutamine (Gln) metabolism between non-muscle-invasive bladder cancer (NMIBC) and muscle-invasive bladder cancer (MIBC). Methods The Oncomine database was searched to analyze the articles on differential expression of glutaminase (Gls) between NIMIBC and MIBC. The expression levels of Gls in NMIBC and MIBC tissues were determined by immunohistochemistry. MTT assay, Western Blot, and cell apoptosis assay were used to analyze and compare the expression of Gls protein, cell proliferation, and apoptosis level in bladder cancer cell lines T24 and 5637, which had different degrees of Gln dependence, in the presence or absence of Gln. Results The MIBC tissues had higher expression of Gls than NMIBC tissues. Gls expression level was different between T24 and 5637 cells, which had different degrees of Gln dependence. For Gln-dependent T24 cells, higher concentration of Gln was associated with higher expression of Gls, higher cell proliferation rate, and less cell apoptosis. Conclusion There is a difference between NMIBC and MIBC regarding dependence on Gln metabolism. Gln deficiency may affect the survival of MIBC by promoting the apoptosis of tumor cells
