Simultaneous determination of chlorogenic and isochlorogenic acid contents in Xiao'er Yanbian granule by HPLC.

The contents of chlorogenic acid and isochlorogenic acid A in Xiao'er Yanbian Granule were determined simultaneously by Reversed-Phase HPLC with acetonitrile-methanol-0.1% phosphoric acid (20:6.5:73.5) as the mobile phase. Investigation of the injection amounts of the two types of chlorogenic acids by the methodology revealed that the injection amounts were in good linearity with the peak areas at a range of chlorogenic acid injection amount of 0.050~1.250 μg, and a isochlorogenic acid A injection amount range of 0.025~0.625 μg. The average recovery (n = 9) was 98.59% for chlorogenic acid, and 100.53% for isochlorogenic acid A, with RSDs of 0.42% and 1.17%, respectively. The contents of chlorogenic acid and isochlorogenic acid A in Xiao'er Yanbian Granule were determined to be 0.78 mg/g and 0.15 mg/g, respectively, this quantification method can be used for the comprehensive evaluation of the quality of Xiao'er Yanbian Granule