Association of adiponectin gene 276G>T polymorphism with polycystic ovary syndrome in Pakistani women.

？Objective: To investigate whether there is association of Adiponectin gene 276G>T polymorphism with the risks of polycystic ovary syndrome (PCOS) in selected Pakistani population. Study design: This study included samples from 106 study subjects, out of which 74 were diagnosed with PCOS and 34 were normal controls). The biochemical, Physical and clinical parameters which are assayed for the samples included menstrual problem, Past and present medical and family history, BMI, waist and hip ratio, LH, FSH, TSH and pro-lactin. Single nucleotide polymorphisms of the adiponectin gene 276 (G>T) were analysed using a PCR-RFLP method. Determination of allelic and genotypic variation with the PCOS was carried out by statistical analysis. Results: Results showed no association of adiponectin gene 276G>T polymorphism with the polycystic ovary syndrome (PCOS) because non-significant difference was found between distributions of genotypes and alleles of both polymorphisms in women with PCOS and controls. Levels of LH, FSH, TSH and prolactin were also analysed, and the results showed significant decline in FSH levels and increase in LH levels in PCOS females comparing with controls. A slightly significant increase was also observed in the serum levels of TSH and prolactin in PCOS women when compared with control. Conclusion: Study suggested that obesity plays an important role in hormonal disturbances that are associated with PCOS. Polymorphism at +276 position of adiponectin gene is not associated with the PCOS and no significant link was observed