Extraction and characterization of ethanolic leaf extracts from Borreria hispida for cytotoxicity applications

The ethanolic leaf extract was collected from Borreria hispida using five different solvents by using shindai technique. The leaf extract contained total phenol and flavonoids with 33.21 ± 0.6 mg GAE and 18.27 ± 0.08 mg QE/g, respectively which is crucial for the potential anticancer activity. The anti-oxidant and anticancer activity was performed by DPPH assay and MTT assay, respectively. The ethanolic leaf extract showed significant amount of phytochemicals with highest (81.3 ± 0.1%) radical scavenging activity. The most potent anticancer activity of ethanolic leaf extract was observed with an IC50 value of 150 μg/ml. Cytotoxicity behaviour of ethanolic leaf extract against Hep cell line were demonstrated elaborately