Biosynthesis and Validation of SNPs from Nymphaea caerulea Savigny

Biosynthesis of nanoparticles is under explorations because of wide biomedical applications and research interest in nanotechnology. Silver nanoparticles have been recently used for a wide range of application including health. The present study is aimed to synthesize silver nanoparticles from leaf extract of Nymphaea caerulea. Stable SNPs were produced after treating the extract with 1mM Ag (NO3)2 solution. A change of thick green color from dark brown was observed. The synthesized Ag NPs were monitored by UV-VIS spectroscopy, SEM, EDAX, AFM and FTIR for their size and shape. The poly dispersed particles having a spherical shape with the range of diameter from 20 to 100 nm. These nanoparticles exhibited high antibacterial effect on the growth of E. coli. It has been demonstrated that the leaf SNPs of N. caerulea are capable of producing silver nanoparticles having important advantage over conventional antibiotics