Effect of phytochemicals and antioxidant compounds enriched extract from CalocybeIndica var. APK2 on proliferation of human MCF-7 breast carcinoma cells

The present study was conducted to evaluate the antiproliferative activity of aqueous extract of Calocybe indica Var. APK2. Mushroom extract was screened for the presence of phytochemicals and antioxidant compounds. The extract was found to contain significant amount of phenolics (7.22 mg GAE/g), flavanoid (1.54 mg quercetin Equivalent/g ), saponin (0.076/g), terpenoid(0.099mg/g) and alkaloid (2.15 mg/g). Mushroom extract analysed for enzymatic antioxidants was found to contain superoxide dismutase (625.27μg/g); catalase (352.8 μg/g); glutathione S transferase (825.26 μg/g); glutathione peroxidase (756.41μg/g) and glucose 6 phosphatase (0.19 μg/g) and. The non enzymatic antioxidant compounds like β-carotene (112.73 μg/100g) and lycopene (22.42 μg/100g) respectively. The above listed compounds enriched extract of the mushroom C.indica was subjected to assess the antiproliferative activity against MCF-7 breast cancer cell line using MTT assay and Tryphan blue dye exclusion method. Results on MTT assay showed that as the concentration increased from 0.125 to 1.0 mg/ml; cell viability decreased from 81.13±0.22% to 29.72±0.02% and 64.31±0.44% to 18.22 ±0.85% at 24h and 48h of incubation respctively. Re sults on Tryphan blue dye exclusion study revealed that the total cell count of MCF-7 cells decreased with increase in concentration of the mushroom extract indicating the inhibitory activity. All these findings point out for the first time that the mushroom, Calocybe indica is an excellent source of natural chemopreventive agents in the treatment of breast cancer