基于酸性微环境研究养阴扶正方对肺癌小鼠体内HIF-1α、Arg1表达的影响
A Study of the Effects of Yangyin Fuzheng Decoction on HIF-1α and Arg1 Expression in Lung Cancer Mice Based on Acidic Microenvironment

目的：探讨养阴扶正方对肺癌小鼠体内HIF-1α、Arg1表达的影响。方法：建立Lewis肺癌C57BL/6鼠移植瘤模型，建模成功后，分别予以养阴扶正方低、中、高剂量组进行干预；绘制肿瘤生长曲线，计算抑瘤率，Elisa方法检测瘤组织中乳酸的含量，West-blot、Real-time分别检测Lewis肺癌HIF-1α、Arg1的蛋白及mRNA表达。结果：Lewis肺癌移植瘤模型建立；养阴扶正方可抑制Lewis肺癌生长，降低Lewis肺癌乳酸，减瘤组织HIF-1α、Arg1的mRNA的表达。结论：养阴扶正方改善了肺癌的酸性微环境，通过对HIF-1α、Arg1的调控，减少M2型TAMs的数量，降低免疫抑制蛋白因子的分泌，逆转了免疫抑制，抑制了肺癌的生长，是养阴扶正方抗NSCLC的新机制。
Objective: Discuss the effects of Yangyin Fuzheng Decoction on HIF-1α and Arg1 expression in lung cancer mice. Method: Establish the model of Lewis lung cancer C57BL/6 mouse transplantation tumor; the low, medium and high dose of Yangyin Fuzheng Decoction were given for intervention after modeling successfully. Draw the tumor growth curve, calculate tumor-inhibition rate, detect the lactic acid content of with Elisa, test HIF-1α, Arg1 protein and mRNA expression of Lewis lung cancer with West-blot and Real-time respectively. Result: The establishment of Lewis lung cancer transplantation tumor model establishment; Yangyin Fuzheng Decoction can inhibit the growth of Lewis lung cancer, decrease Lewis lung cancer lactic acid and reduce mRNA expression of tumor tissue HIF-1αand Arg1. Conclusion: Yangyin Fuzheng Decoction improves the acidic microenvironment of lung cancer by regulating HIF-1αand Arg1, reduces the amount of M2 style TAMs, decreases the secretion of immunosuppressive protein factor, reverses the immunosuppression and inhibits the growth of lung cancer, which is an Anti-NSCLC new mechanism of Yangyin Fuzheng Decoction.