Objectives:
To explore the effects of Qi-invigorating herbs on mitochondrial function using
cultured cells and animal organs. Methods:
Using water extracts of Astragali
radix, we investigated the effect of “Qi-invigoration” on M-1
renal cells and mouse organs in-vitro
including total adenylate production (TAP), reactive oxygen species (ROS)
levels, and mitochondrial membrane potential (MMP). We also examined the effect
on antioxidant capacity by conducting an analysis of superoxide dismutase (SOD)
and glutathione (GSH). Results:
1) Astragali radix
increased mitochondrial TAP generation and decreased ROS levels in both mouse
kidney tissues and M-1 renal cells. 2) Astragali radix also significantly increased MMP and GSH
levels in M-1 cells, but in the kidney tissue, there was no significant change in MMP levels and a
decrease in GSH levels. 3) Astragali
radix stimulated TAP levels in the heart, spleen, lung, kidney
and skeletal muscle tissue, which was accompanied by the reduction of ROS. 4)
For the meridian organs that Astragali
radix belongs to, the energy production and antioxidant capacity
were boosted simultaneously. Conclusions:
These results provide new insights for the biochemical basis of Qi-invigoration and
the meridian tropism theory
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