The lipid composition in terms of the amount of
neutral lipids, free fatty acids and polar lipid content is of great importance
to make full use possible of this fraction and to define the suitability of its
application, either as a raw material for fuel production, nutraceutical
purposes or feed. In addition to the fatty components present in the lipid
extract, other components may be included, such as: carotenoids, pigments and
sterols. The microalgae studied in this work, Scenedesmus sp. and Desmodesmus sp., were subjected to the same growth conditions and evaluated for lipid
content, quantification and diversity of lipid components as well as its fatty
acid profile. For lipid determination two extraction methods were compared: the J:Schmid-Bondzynski-Ratzlaff and Bligh & Dyer method. For Desmodesmus sp. 5.43% ± 0.41% and 9.18% ± 0.33% of lipids were
obtained on an ash-free dry weight basis and for Scenedesmus sp. 12.46% ± 0.38% and 8.16% ± 0.42% of lipids were
obtained on an？ash-free dry weight basis using for both methods J:Schmid-Bondzynski-Ratzlaff and Bligh & Dyer, respectively. For the
identification of the main lipid components present in the extracts, the Thin
layer chromatography (TLC) technique was used. This made it possible,
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