Medicinal plants have been used to treat many diseases with fewer side effects compared to clinical medicines. The need for treatment by medicinal plants has been increasing in response to the accumulation of environmental pollutants. The herbicide 2, 4-Dichlorophenoxyacetic acid has been considered as fast spreadable environmental pollutant, which may cause many disorders in animals and possibly in humans. The aim of this study is to assess the protective and/or curative effect of Lepidium sativum against hepatotoxicity induced by 2, 4-D herbicide in rats. 25 Albino Wistar rats were grouped into 4 groups: a control group, the curative group treated for 4 weeks with LS water extract after a single dose 2, 4-D LD50 administration, protective group which was treated for 4 weeks with LS water extract prior to the 2, 4-D LD50 administration, and LS only group that was given the LS water extract as a positive control. Using transmission electron microscope, the histological features showed that curative group hepatocytes still suffered from focal necrosis and vacuolation of hepatocytes as a result of 2, 4-D toxic effect whereas in the protective group hepatocytes were less accentuated expressed as less frequent necrotic area, and still large mitochondria were present with numerous undissolved lipid droplets and a small amount of rough endoplasmic reticulum. For the LS only group, nearly normal cells with centric nucleus, normal small mitochondria were at a normal nucleus-cytoplasmic ratio, but a few lipid droplets were noticed. This study proved that curative group has an advantage over the protective group that could be presented in the nucleus with narrow nuclear pores and intact nuclear envelope with dense small mitochondria and normal rough endoplasmic reticulum that were recorded as in the control group. Thus, Lepidium sativum administration to the rats prior or post to intoxication was effective in minimizing the 2, 4-D-induced damages likely through a free radical scavenging activity in liver tissue.
References
[1]
Coelho, J., Barros, L., Dias, M., Finimundy, T., Amaral, J., Alves, M., Calhelha, R., Santos, P. and Ferreira, I. (2020) Echinacea purpurea (L.) Moench: Chemical Characterization and Bioactivity of Its Extracts and Fractions. Pharmaceuticals, 13, 125-141. https://doi.org/10.3390/ph13060125
[2]
El-Huneidi, W., Shehab, N.G., Bajbouj, K., Vinod, A., El-Serafi, A., Shafarin, J., Bou Malhab, L.J., Abdel-Rahman, W.M. and Abu-Gharbieh, E. (2020) Micromeria fruticosa Induces Cell Cycle Arrest and Apoptosis in Breast and Colorectal Cancer Cells. Pharmaceuticals, 13, 115-126. https://doi.org/10.3390/ph13060115
[3]
Rahman, M.A., Mossa, J.S., Al-Said, M.S. and Al-Yahya, M.A. (2004) Medicinal Plant Diversity in the Flora of Saudi Arabia 1: A Report on Seven Plant Families. Fitoterapia, 75, 149-161. https://doi.org/10.1016/j.fitote.2003.12.012
[4]
Golkar, F.H. and Koohi Dehkordi, M. (2018) Production of a New Mucilage Compound in Lepidium sativum Callus by Optimizing in Vitro Growth Conditions. Natural Product Research, 33, 130-135. https://doi.org/10.1080/14786419.2018.1437426
[5]
Merzouki, A., Ed-derfoufi, F. and Mesa, J.M. (2000) Contribution to the Knowledge of Rifian Traditional Medicine. II: Folk Medicine in Ksar Lakbir District (NW Morocco). Fitoterapia, 71, 278-307. https://doi.org/10.1016/S0367-326X(00)00139-8
[6]
Nadkarini, A.K. (1954) Indian Material Medica. Popular Prakashan Pvt. Ltd., Bombay, 736-737.
[7]
Eddouks, M., Maghrani, M., Zeggwagh, N.A. and Michel, J.B. (2005) Study of the Hypoglycemic Activity of Lepidium sativum L. Aqueous Extract in Normal and Diabetic Rats. Journal of Ethnopharmacology, 97, 391-395. https://doi.org/10.1016/j.jep.2004.11.030
[8]
Maghrani, M., Zegwash, N.A., Michel, J.B. and Eddouks, M. (2005) Antihypertensive Activity of Lepidium sativum L, in Spontaneously Hypertensive Rats. Journal of Ethnopharmacology, 100, 193-197. https://doi.org/10.1016/j.jep.2005.02.024
[9]
Mali, R.G., Mahajan, S.G. and Mehta, A.A. (2007) Lepidium sativum (Garden Cress): A Review of Contemporary Literature and Medicinal Properties. Oriental Pharmacy and Experimental Medicine, 7, 331-335. https://doi.org/10.3742/OPEM.2007.7.4.331
[10]
Juma, A.B. (2007) The Effect of Lepidium sativum Seeds on Fracture Induced Healing in Rabbits. Medscape General Medicine, 9, 23-37.
[11]
Dakhakhni, T.H., Raouf, G.A. and Ali A. (2020) Investigation of 2, 4-D Hepatotoxicity and Lipidium Sativum Antitoxicity on Rat Tissue Using FT-IR Spectroscopy. International Journal of Animal Biotechnology and Applications, 6, 17-29.
[12]
Antoine, K.M., Mortazavi, S., Miller, A.D. and Miller, L.M. (2010) Chemical Differences Are Observed in Children’s versus Adults’ Latent Fingerprints as a Function of Time. Journal of Forensic Sciences, 55, 513-518.
[13]
Fahey, J.W., Zalcmann, A.T. and Talalay, P. (2015) The Chemical Diversity and Distribution of Glucosinolates and Isothiocyanates among Plants. Phytochemistry, 56, 5-51. https://doi.org/10.1016/S0031-9422(00)00316-2
[14]
Abdallah, H.M., Farag, M.A., Algandaby, M.M., Nasrullah, M.Z., Abdel-Naim, A.B., Eid, B.G., Safo, M.K., Koshak, A.E. and Malebar A.M. (2020) Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats. Nutrients, 12, 2075-2095. https://doi.org/10.3390/nu12072075
[15]
Kamani, M., Hosseini, E.S., Kashani, H.H., Atlasi, M.A. and Nikzad, H. (2017) Protective Effect of Lepidium sativum Seed Extract on Histopathology and Morphology of Epididymis in Diabetic rat Model. International Journal of Morphology, 35, 603-610. http://dx.doi.org/10.4067/S0717-9502201700020003
[16]
Islam, F., Wang, J., Farooq, M.A., Khan, M.S.S., Xu, L., Zhu, J.W., Zhao, M., Munos, S., Li, Q.X. and Zhou, W.J. (2018) Potential Impact of the Herbicide 2, 4-Dichlorophenoxyacetic Acid on Human and Ecosystems. Environment International, 111, 332-351. https://doi.org/10.1016/j.envint.2017.10.020
[17]
Thiel, N.A., Sachett, A., Schneider, S.E., Garbinato, C., Decui, L., Eichwald, T., et al. (2020) Exposure to the Herbicide 2,4-Dichlorophenoxyacetic Acid Impairs Mitochondrial Function, Oxidative Status, and Behavior in Adult Zebrafish. Environmental Science and Pollution Research, 27, 45874-45882. https://doi.org/10.1007/s11356-020-10497-6
[18]
Tichati, L., Trea, F. and Oual, K. (2020) Potential Role of Selenium against Hepatotoxicity Induced by 2,4-Dichlorophenoxyacetic Acid in Albino Wistar Rats. Biological Trace Element Research, 194, 228-236. https://doi.org/10.1007/s12011-019-01773-9
[19]
Evangelista de Duffard, A.M., Fabra de Peretti, A., Castro de Cantarini, S. and Duffard, R. (1993) Effects of 2, 4-Dichlorophenoxyacetic Acid Butyl Ester on Chick Liver. Archives of Environmental Contamination and Toxicology, 25, 204-211. https://doi.org/10.1007/BF00212131
[20]
Troudi, A., Ben Amara, I., Samet, A.M. and Zeghal, N. (2012) Oxidative Stress Induced by 2,4-Phenoxyacetic Acid in Liver of Female Rats and Their Progeny: Biochemical and Histopathological Studies. Environmental Toxicology, 27, 137-145.
[21]
Dakhakhni, T.H., Raouf, G.A. and Ali, A. (2016) Evaluation of the Toxic effect of the Herbicide 2,4-D on Rat Hepatocytes: an FT-IR Spectroscopic Study. European Biophysics Journal, 45, 311-320. https://doi.org/10.1007/s00249-015-1097-7
[22]
Harada, Y., Tanaka, N., Ichikawa, M., Kamijo, Y., Sugiyama, E., Gonzalez, F.J. and Aoyama, T. (2016) PPARα-Dependent Cholesterol/Testosterone Disruption in Leydig Cells Mediates 2,4-Dichlorophenoxyacetic Acid-Induced Testicular Toxicity in Mice. Archives of Toxicology, 90, 3061-3071. https://doi.org/10.1007/s00204-016-1669-z
[23]
Lerda, D. and Rizzi, R. (1991) Study of Reproductive Function in Persons Occupationally Exposed to 2,4-Dichlorophenoxyacetic Acid (2,4-D). Mutation Research Letters, 262, 47-50. https://doi.org/10.1016/0165-7992(91)90105-D
[24]
Amel, N., Wafa, T., Samia, D., Yousra, D., Issam, C., Attia, N. and Mohamed, H. (2016) Extra Virgin Olive Oil Modulates Brain Docosahexaenoic Acid Level and Oxidative Damage Caused by 2,4-Dichlorophenoxyacetic Acid in Rats. Journal of Food Science and Technology, 53, 1454-1464. https://doi.org/10.1007/s13197-015-2150-3
[25]
United States Environmental Protection Association (2005) Reregistration Eligibility Decision for 2,4-D. United States Environmental Protection Association, New York.
[26]
Willcox, J.K., Ash, S.L. and Catignani, G.L. (2004) Antioxidants and Prevention of Chronic Disease. Critical Reviews in Food Science and Nutrition, 44, 275-295. https://doi.org/10.1080/10408690490468489
[27]
Akkas, S.B., Severcan, M., Yilmaz, O. and Severcan, F. (2007) Effects of Lipoic Acid Supplementation on Rat Brain Tissue: An FTIR Spectroscopic and Neural Network Study. Food Chemistry, 105, 1281-1288. https://doi.org/10.1016/j.foodchem.2007.03.015
[28]
Robin, M., Sauvage, I., Grandperret, T., Descatoire, V., Pessayre, D. and Fromenty, B. (2005) Ethanol Increases Mitochondrial Cytochrom p450 in Mouse Liver and Rat Hepatocytes. FEBS Letters, 579, 6895-6902.
