The beginning of human life under time-laps Cinematography

Sa？etak Background and Purpose: The aim of this study was to follow and record the early stages of development of a human embryo using time-lapse cinematography, and to discuss the beginning of human life. Materials and Methods: Human ova were incubated in thermo-stabilized and pH controlled chambers and observed using an inverted microscope fitted with a CCD digital camera. Images were taken at 2-minute intervals for 5–6 days. A total of 30 consecutive images, corresponding to an hour of incubation, could then be replayed in 1 second. This allowed us to watch the development of embryos over 5–6 days in a few minutes. Results and Conclusion: We observed fertilization of an ovum with a single spermatozoon followed by early cleavages, formation of the morula, blastocyst hatching and changes in the embryonic plates and the development of monozygotic twins in 2 of 3 incubated blastocysts. TLC has contributed enormously to our understanding of the morphological mechanisms of fertilization and embryonic development.We would like to suggest that the time at which stem cell-like morula cells form the differentiated blastocyst is the point at which human life begins, and that the ET of blastocyst may increase the risk of monozygotic twins