Determination of Dazomet in Basamid Granulat Using Reversed Phase HPLC

Sa？etak The reverse phase HPLC determination of dazomet (tetrahydro-3,5-dimethyl-1,3,5-thiadiazine-2-tione) in a pesticide formulation has been studied. HS Pecosphere 3 x 3 C8 (3 μm, 3.3 x 0.46 cm) and LiChrosorb C8 (5 μm, 25 x 0.4 cm) analytical columns were tested at a flow rate of 1.0 cm3 min-1, column temperature of 25 °C and UV detection at 280 nm. The best separation of dazomet from its dehydro-dimer forms was achieved with a mobile phase containing acetonitrile-water in the volume ratio 15 : 85 on a HS Pecosphere column, and acetonitrile-water in the volume ratio 30 : 70 on a LiChrosorb C8 column. The HS Pecosphere column showed a better peak symmetry, separation factor, and multiple correlation coefficient. The retention time and peak area for the HS Pecosphere column were precise within a day and between days as indicated by the ANO VA test, in contrast to the LiChrosorb column, which showed lost of efficiency