Langmuir Aggregation of Dahliaviolett on DNA

Sa？etak Formation of double electrostatic films in DNA causes Langmuir aggregation of small ions and molecules only in a monolayer. We studied the interaction of dahliaviolett (DLV) with DNA at pH = 7.24 by a spectral correction technique. Results showed that the ad-sorption ratio of DLV to DNA-P was 1 : 4, the adsorption constant of the DLV-DNA aggregate was 4.44 x 104 and its molar absorption coefficient was 1.02 x 105 dm3 mol-1 cm-1 at 615 nm. This aggregation was applied to the quantitative determination of DNA in samples