Isolation and Characterization of Beneficial Microorganisms in Organic, Semi-Organic and Conventional Fertilizer Treated Agricultural Field Soil and Comparison of Bacterial Richness
The effect of different farming systems on microbial communities in agricultural environment was investigated in the present study. Depending on the present farming trend,？the microbial distribution in agricultural soils treated with organic, semi-organic and conventional fertilizers was analyzed. A total of 20 soil samples were collected from different types of agricultural fields of Bangladesh Agricultural Research Institute (BARI, Gazipur). Microorganisms playing beneficial roles in soil such as nitrogen fixation (e.g. Rhizobium sp.,？Azotobacter sp.), phosphate solubilization (e.g. Bacillus sp.,？Pseudomonas sp.,？Phosphobacteria) and auxin production (e.g. Pseudomonas sp.,？Serratia sp.？and Bacillus sp.) were evaluated from each of the samples. The results revealed that agricultural fields treated with chemical fertilizers showed lower microbial count than that of organic fertilizer treated agricultural fields’ soil samples. In addition, organic fertilizers amended field soils have higher phytohormone (Auxin) activities, phosphate solubilization bacteria and other bacterial richness compared to chemical fertilizer applied field soil.
References
[1]
Jenkinson, D.S. and Ladd, J.N. (1981) Microbial Biomass in Soil Measurement and Turnover. In: Paul, E.A. and Ladd, J.N., Eds., Soil Biochemistry, Vol. 5, Marcel Dekker Inc., New York and Basel, 415-471.
[2]
Smith, J. and Paul, E. (1990) The Significance of Soil Microbial Biomass Estimations: Soil.
[3]
Leita, L., De Nobili, M., Mondini, C., Muhlbachova, G., Marchiol, L., Bragato, G. and Contin, M. (1999) Influence of Inorganic and Organic Fertilization on Soil Microbial Biomass, Metabolic Quotient and Heavy Metal Bioavailability. Biology and Fertility of Soils, 28, 371-376. https://doi.org/10.1007/s003740050506
[4]
Kaur, T., Brar, B. and Dhillon, N. (2008) Soil Organic Matter Dynamics as Affected by Long-Term Use of Organic and Inorganic Fertilizers under Maize-Wheat Cropping System. Nutrient Cycling in Agroecosystems, 81, 59-69. https://doi.org/10.1007/s10705-007-9152-0
[5]
Chaudhry, A., Jilani, G., Khan, M. and Iqbal, T. (2009) Improved Processing of Poultry Litter Reduces Nitrate Leaching and Enhances Its Fertilizer Quality. Asian journal of Chemistry, 21, 4997-5003.
[6]
Leite, L.F., Oliveira, F.C., Araújo, A.S., Galvão, S.R., Lemos, J.O. and Silva, E.F. (2010) Soil Organic Carbon and Biological Indicators in an Acrisol under Tillage Systems and Organic Management in North-Eastern Brazil. Soil Research, 48, 258-265. https://doi.org/10.1071/SR09122
[7]
Rasul, G. and Thapa, G.B. (2004) Sustainability of Ecological and Conventional Agricultural Systems in Bangladesh: An Assessment Based on Environmental, Economic and Social Perspectives. Agricultural Systems, 79, 327-351. https://doi.org/10.1016/S0308-521X(03)00090-8
[8]
Asaduzzaman, M. (1996) Resource Degradation and Sustainable Development in Bangladesh: Some Preliminary Estimates. Seminar on Planning for Sustainable Development of Bangladesh, Bangladesh Institute of Development Studies, Dhaka, 24-25 September 1996.
[9]
Hossain, S. and Kashem, M. (1997) Agronomic Management to Combat Declining Soil Fertility in Bangladesh. 6th Biennial Conference of the Bangladesh Society of Agronomy, Dhaka, 29 July 1997.
[10]
Hossain, M., Naher, F. and Shahabuddin, Q. (2005) Food Security and Nutrition in Bangladesh: Progress and Determinants. eJADE: Electronic Journal of Agricultural and Development Economics, 2, 103-132.
[11]
Gowsalya, A., Ponnusami, V. and Sugumaran, K. (2014) Isolation of Bacteria from Soil Sample for Exo-Polysaccharide Production. International Journal of ChemTech Research, 6, 2925-2928.
[12]
Al-Mujahidy, S.M.J., Hassan, M., Rahman, M. and Mamun-Or-Rashid, A. (2013) Isolation and Characterization of Rhizobium spp. and Determination of Their Potency for Growth Factor Production. International Research Journal of Biotechnology, 47, 117-123.
[13]
Warghane, A.J., Wagh, G., Nag, B., SP, J.M., Thaware, R. and Kitey, H. (2011) Isolation and Characterization of Pseudomonas Species from Godavari River Sample. Asiatic Journal of Biotechnology Resources, 2, 862-866.
[14]
Kizilkaya, R. (2009) Nitrogen Fixation Capacity of Azotobacter spp. Strains Isolated from Soils in Different Ecosystems and Relationship between Them and the Microbiological Properties of Soils. Journal of Environmental Biology, 30, 73-82.
[15]
Viruel, E., Lucca, M.E. and Siñeriz, F. (2011) Plant Growth Promotion Traits of Phosphobacteria Isolated from Puna, Argentina. Archives of Microbiology, 193, 489-496. https://doi.org/10.1007/s00203-011-0692-y
[16]
Rohilla, S. and Salar, R. (2012) Isolation and Characterization of Various Fungal Strains from Agricultural Soil Contaminated with Pesticides. Research Journal of Recent Sciences, 2277, 2502.
[17]
Marti, R., Scott, A., Tien, Y.-C., Murray, R., Sabourin, L., Zhang, Y. and Topp, E. (2013) Impact of Manure Fertilization on the Abundance of Antibiotic-Resistant Bacteria and Frequency of Detection of Antibiotic Resistance Genes in Soil and on Vegetables at Harvest. Applied and Environmental Microbiology, 79, 5701-5709. https://doi.org/10.1128/AEM.01682-13
[18]
Ehmann, A. (1977) The Van Urk-Salkowski Reagent—A Sensitive and Specific Chromogenic Reagent for Silica Gel Thin-Layer Chromatographic Detection and Identification of Indole Derivatives. Journal of Chromatography A, 132, 267-276. https://doi.org/10.1016/S0021-9673(00)89300-0
[19]
Mohite, B. (2013) Isolation and Characterization of Indole Acetic Acid (IAA) Producing Bacteria from Rhizospheric Soil and Its Effect on Plant Growth. Journal of Soil Science and Plant Nutrition, 13, 638-649. https://doi.org/10.4067/S0718-95162013005000051
[20]
Walpola, B.C. and Arunakumara, K. (2016) Assessment of Phosphate Solubilization and Indole Acetic Acid Production in Plant Growth Promoting Bacteria Isolated from Green House Soils of Gonju-Gun, South Korea. Tropical Agricultural Research and Extension, 18, 31-39. https://doi.org/10.4038/tare.v18i1.5322
[21]
Bohloli Khiavi, R. (2017) Methods for in Vitro Evaluating Antimicrobial Activity: A Review. Laboratory & Diagnosis, 9, 43-53.
[22]
Kalayu, G. (2019) Phosphate Solubilizing Microorganisms: Promising Approach as Biofertilizers. International Journal of Agronomy, 2019, Article ID: 4917256. https://doi.org/10.1155/2019/4917256
[23]
Ahmad, F., Ahmad, I. and Khan, M.S. (2005) Indole Acetic Acid Production by the Indigenous Isolates of Azotobacter and Fluorescent Pseudomonas in the Presence and Absence of Tryptophan. Turkish Journal of Biology, 29, 29-34.
[24]
Sarwar, M. and Kremer, R. (1995) Determination of Bacterially Derived Auxins Using a Microplate Method. Letters in Applied Microbiology, 20, 282-285. https://doi.org/10.1111/j.1472-765X.1995.tb00446.x
[25]
Lwin, K.M., Myint, M.M., Tar, T. and Aung, W.Z.M. (2012) Isolation of Plant Hormone (Indole-3-Acetic Acid-IAA) Producing Rhizobacteria and Study on Their Effects on Maize Seedling. Engineering Journal, 16, 137-144. https://doi.org/10.4186/ej.2012.16.5.137
[26]
Vaghasia, H.L., Patel, G.M., Chudasama, R.S. and Bhatt, K.R. (2011) Screening of IAA from Rhizospher Microflora of Field Crops. Bioscience Discovery Journal, 2, 94-100.
[27]
Tien, T., Gaskins, M. and Hubbell, D. (1979) Plant Growth Substances Produced by Azospirillum brasilense and Their Effect on the Growth of Pearl Millet (Pennisetum americanum L.). Applied and Environmental Microbiology, 37, 1016-1024. https://doi.org/10.1128/AEM.37.5.1016-1024.1979
[28]
Karnwal, A. (2009) Production of Indole Acetic Acid by Fluorescent Pseudomonas in the Presence of L-Tryptophan and Rice Root Exudates. Journal of Plant Pathology, 91, 61-63.
[29]
Wani, P.A., Khan, M.S. and Zaidi, A. (2007) Synergistic Effects of the Inoculation with Nitrogen-Fixing and Phosphate-Solubilizing Rhizobacteria on the Performance of Field-Grown Chickpea. Journal of Plant Nutrition and Soil Science, 170, 283-287. https://doi.org/10.1002/jpln.200620602
[30]
Wakelin, S.A., Warren, R.A., Harvey, P.R. and Ryder, M.H. (2004) Phosphate Solubilization by Penicillium spp. Closely Associated with Wheat Roots. Biology and Fertility of Soils, 40, 36-43. https://doi.org/10.1007/s00374-004-0750-6
[31]
Saxena, J., Basu, P. and Jaligam, V. (2013) Phosphate Solubilization by a Few Fungal Strains Belonging to the Genera Aspergillus and Penicillium. African Journal of Microbiology Research, 7, 4862-4869. https://doi.org/10.5897/AJMR2013.5991
[32]
Hrudayanath, T., Bikash, C., Rashmi, R. and Sushil, K. (2013) Biodiversity and Biotechnological Potential of Microorganisms from Mangrove Ecosystem: A Review. Annals of Microbiology, 63, 1-19.
[33]
Bertazzi, P.A., Consonni, D., Bachetti, S., Rubagotti, M., Baccarelli, A., Zocchetti, C. and Pesatori, A.C. (2001) Health Effects of Dioxin Exposure: A 20-Year Mortality Study. American Journal of Epidemiology, 153, 1031-1044. https://doi.org/10.1093/aje/153.11.1031
[34]
Waskom, R.M. and Yergert, M.D. (1994) Best Management Practices for Pesticide and Fertilizer Storage and Handling. Bulletin (Colorado State University Cooperative Extension Service), XCM-178.
[35]
Ramirez, K.S., Craine, J.M. and Fierer, N. (2010) Nitrogen Fertilization Inhibits Soil Microbial Respiration Regardless of the Form of Nitrogen Applied. Soil Biology and Biochemistry, 42, 2336-2338. https://doi.org/10.1016/j.soilbio.2010.08.032
[36]
Yadav, S., Babu, S., Yadav, M., Singh, K., Yadav, G. and Pal, S. (2013) A Review of Organic Farming for Sustainable Agriculture in Northern India. International Journal of Agronomy, 2013, Article ID: 718145. https://doi.org/10.1155/2013/718145
[37]
Bagali, A., Patil, H., Chimmad, V., Patil, P. and Patil, R. (2012) Effect of Inorganics and Organics on Growth and Yield of Onion (Allium cepa L.). Karnataka Journal of Agricultural Sciences, 25, 112-115.
[38]
Rashid, M., Khalil, S., Ayub, N., Alam, S. and Latif, F. (2004) Organic Acids Production and Phosphate Solubilization by Phosphate Solubilizing Microorganisms (PSM) under in Vitro Conditions. Pakistan Journal of Biological Sciences, 7, 187-196. https://doi.org/10.3923/pjbs.2004.187.196
[39]
Leggett, M., Newlands, N., Greenshields, D., West, L., Inman, S. and Koivunen, M. (2015) Maize Yield Response to a Phosphorus-Solubilizing Microbial Inoculant in Field Trials. The Journal of Agricultural Science, 153, 1464-1478. https://doi.org/10.1017/S0021859614001166
[40]
Marra, L.M., Oliveira, S.Md., Soares, C.R.F.S. and Moreira, F.Md.S. (2011) Solubilisation of Inorganic Phosphates by Inoculant Strains from Tropical Legumes. Scientia Agricola, 68, 603-609. https://doi.org/10.1590/S0103-90162011000500015
[41]
Glick, B.R., Cheng, Z., Czarny, J. and Duan, J. (2007) Promotion of Plant Growth by ACC Deaminase-Producing Soil Bacteria. In: New Perspectives and Approaches in Plant Growth-Promoting Rhizobacteria Research, Springer, Berlin, 329-339. https://doi.org/10.1007/978-1-4020-6776-1_8
[42]
Khan, M.S., Zaidi, A. and Ahmad, E. (2014) Mechanism of Phosphate Solubilization and Physiological Functions of Phosphate-Solubilizing Microorganisms. In: Phosphate Solubilizing Microorganisms, Springer, Berlin, 31-62. https://doi.org/10.1007/978-3-319-08216-5_2
[43]
Rai, N., Ashiya, P. and Rathore, D.S. (2014) Comparative Study of the Effect of Chemical Fertilizers and Organic Fertilizers on Eisenia foetida. International Journal of Innovative Research in Science, 3, 12991-12998.
[44]
Hansen, B., Alrøe, H.F. and Kristensen, E.S. (2001) Approaches to Assess the Environmental Impact of Organic Farming with Particular Regard to Denmark. Agriculture, Ecosystems & Environment, 83, 11-26. https://doi.org/10.1016/S0167-8809(00)00257-7
[45]
Stolze, M., Piorr, A., Häring, A.M. and Dabbert, S. (2000) Environmental Impacts of Organic Farming in Europe. Universität Hohenheim, Stuttgart-Hohenheim.
[46]
Pulleman, M., Jongmans, A., Marinissen, J. and Bouma, J. (2003) Effects of Organic versus Conventional Arable Farming on Soil Structure and Organic Matter Dynamics in a Marine Loam in the Netherlands. Soil Use and Management, 19, 157-165. https://doi.org/10.1079/SUM2003186
[47]
Oehl, F., Sieverding, E., Mäder, P., Dubois, D., Ineichen, K., Boller, T. and Wiemken, A. (2004) Impact of Long-Term Conventional and Organic Farming on the Diversity of Arbuscular Mycorrhizal Fungi. Oecologia, 138, 574-583. https://doi.org/10.1007/s00442-003-1458-2
[48]
Mäder, P., Fliessbach, A., Dubois, D., Gunst, L., Fried, P. and Niggli, U. (2002) Soil Fertility and Biodiversity in Organic Farming. Science (New York, NY), 296, 1694-1697. https://doi.org/10.1126/science.1071148
[49]
Morris, M., Kelly, V.A., Kopicki, R.J. and Byerlee, D. (2007) Fertilizer Use in African Agriculture: Lessons Learned and Good Practice Guidelines. The World Bank, Washington DC. https://doi.org/10.1596/978-0-8213-6880-0
[50]
Hoque, M.N. (2012) Eco-Friendly and Organic Farming in Bangladesh: International Classification and Local Practice. Universitätsbibliothek.
