We describe a novel, non-invasive peptide-based product intended to counter several pathophysiological signs of aged skin through the topical application of signalling molecule mimics. Several aesthetic parameters of skin physiology were assessed. The product was tested on 20 healthy Caucasian women (35 - 55 years-old) who applied the product twice-daily to the face for 30 days. Skin elasticity, firmness, sagging, brightness, and luminosity were assessed. Additionally, over a period of 3 hours, the hydrating effect of the product vs placebo vs non-treated skin was assessed. After 30 days, participants showed significant increases in skin elasticity (11.8% increase, p < 0.01), luminosity (2.5% increase, p < 0.01), and brightness (110% increase, p < 0.001) with concomitant significant decreases in skin sagging (6.3% decrease, p < 0.05). The product gave a rapid and sustained hydrating action that was significantly greater than placebo alone (10.2% increase, p < 0.05). No adverse reactions were reported. Cosmetic products based on the action of synthetic peptide mimics of endogenous signalling molecules show promise for combatting the pathological degradation of skin as seen in the elderly. Strategies to reverse these symptoms through non-invasive techniques warrant further investigation. This study provides support for the application of peptides to combat signs of aging and to improve the general condition of the skin.
References
[1]
Naval, J., Alonso, V. and Herranz, M.A. (2014) Genetic Polymorphisms and Skin Aging: The Identification of Population Genotypic Groups Holds Potential for Personalized Treatments. Clinical, Cosmetic and Investigational Dermatology, 7, 207-214. https://doi.org/10.2147/CCID.S55669
[2]
Shekar, S.N., Luciano, M., Duffy, D.L. and Martin, N.G. (2005) Genetic and Environmental Influences on Skin Pattern Deterioration. Journal of Investigative Dermatology, 125, 1119-1129. https://doi.org/10.1111/j.0022-202X.2005.23961.x
[3]
Fisher, G.J., et al. (2009) Collagen Fragmentation Promotes Oxidative Stress and Elevates Matrix Metalloproteinase-1 in Fibroblasts in Aged Human Skin. The American Journal of Pathology, 174, 101-114. https://doi.org/10.2353/ajpath.2009.080599
[4]
Panich, U., Sittithumcharee, G., Rathviboon, N. and Jirawatnotai, S. (2016) Ultraviolet Radiation-Induced Skin Aging: The Role of DNA Damage and Oxidative Stress in Epidermal Stem Cell Damage Mediated Skin Aging. Stem Cells International, 2016, Article ID: 7370642. https://doi.org/10.1155/2016/7370642
[5]
Fisher, G.J., Wang, Z.Q., Datta, S.C., Varani, J., Kang, S. and Voorhees, J.J. (1997) Pathophysiology of Premature Skin Aging Induced by Ultraviolet Light. The New England Journal of Medicine, 337, 1419-1428. https://doi.org/10.1056/NEJM199711133372003
[6]
Flament, F., Ye, C. and Amar, D. (2019) Assessing the Impact of an Aerial Chronic Urban Pollution (UP) on Some Facial Signs of Differently-Aged Chinese Men. International Journal of Cosmetic Science, 41, 450-461. https://doi.org/10.1111/ics.12558
[7]
Boyd, A.S., Stasko, T., King, L.E., Cameron, G.S., Pearse, A.D. and Gaskell, S.A. (1999) Cigarette Smoking-Associated Elastotic Changes in the Skin. Journal of the American Academy of Dermatology, 41, 23-26. https://doi.org/10.1016/S0190-9622(99)70400-7
[8]
Fisher, G.J., et al. (2002) Mechanisms of Photoaging and Chronological Skin Aging. Archives of Dermatology, 138, 1462-1470. https://doi.org/10.1001/archderm.138.11.1462
[9]
Katayama, K., Armendariz-Borunda, J., Raghow, R., Kang, A.H. and Seyer, J.M. (1993) A Pentapeptide from Type I Procollagen Promotes Extracellular Matrix Production. Journal of Biological Chemistry, 268, 9941-9944.
[10]
Park, H., An, E. and Cho Lee, A.-R. (2017) Effect of Palmitoyl-Pentapeptide (Pal-KTTKS) on Wound Contractile Process in Relation with Connective Tissue Growth Factor and α-Smooth Muscle Actin Expression. Tissue Engineering and Regenerative Medicine, 14, 73-80. https://doi.org/10.1007/s13770-016-0017-y
[11]
Tsai, W.-C., Hsu, C.-C., Chung, C.-Y., Lin, M.-S., Li, S.-L. and Pang, J.-H.S. (2007) The Pentapeptide KTTKS Promoting the Expressions of Type I Collagen and Transforming Growth Factor-Beta of Tendon Cells. Journal of Orthopaedic Research, 25, 1629-1634. https://doi.org/10.1002/jor.20455
[12]
Aguilar-Toalá, J.E., Hernández-Mendoza, A., González-Córdova, A.F., Vallejo-Cordoba, B. and Liceaga, A.M. (2019) Potential Role of Natural Bioactive Peptides for Development of Cosmeceutical Skin Products. Peptides, 122, Article ID: 170170. https://doi.org/10.1016/j.peptides.2019.170170
[13]
Rodrigues-Barata, A.R. and Camacho-Martínez, F.M. (2013) Undesirable Effects after Treatment with Dermal Fillers. Journal of Drugs in Dermatology, 12, e59-e62.
[14]
Schwarz, A., et al. (2001) Interleukin-12 Suppresses Ultraviolet Radiation-Induced Apoptosis by Inducing DNA Repair. Nature Cell Biology, 4, 26-31. https://doi.org/10.1038/ncb717
[15]
Franzén, L., Ghassemifar, R. and Schultz, G. (1993) Specific Binding of EGF in Connective Tissue Repair. European Journal of Cell Biology, 60, 346-350.
[16]
Amara, H.B., Thoma, D.S., Schwarz, F., Song, H.Y., Capetillo, J. and Koo, K.-T. (2019) Healing Kinetics of Oral Soft Tissue Wounds Treated with Recombinant Epidermal Growth Factor: Translation from a Canine Model. Journal of Clinical Periodontology, 46, 105-117. https://doi.org/10.1111/jcpe.13035
[17]
Esquirol-Caussa, J. and Herrero-Vila, E. (2019) Human Recombinant Epidermal Growth Factor in Skin Lesions: 77 Cases in EPItelizando Project. Journal of Dermatological Treatment, 30, 96-101. https://doi.org/10.1080/09546634.2018.1468546
[18]
Lewis, D.A., Travers, J.B., Somani, A.-K. and Spandau, D.F. (2010) The IGF-1/IGF-1R Signaling Axis in the Skin: A New Role for the Dermis in Aging-Associated Skin Cancer. Oncogene, 29, 1475-1485. https://doi.org/10.1038/onc.2009.440
[19]
Lewis, D.A., Yi, Q., Travers, J.B. and Spandau, D.F. (2008) UVB-Induced Senescence in Human Keratinocytes Requires a Functional Insulin-Like Growth Factor-1 Receptor and p53. Molecular Biology of the Cell, 19, 1346-1353. https://doi.org/10.1091/mbc.e07-10-1041
[20]
Gong, F., et al. (2019) Effect of Insulin-Like Growth Factor-1 on Promoting Healing of Skin Ulcers in Diabetic Rats. Journal of Biological Regulators and Homeostatic Agents, 33, 687-694.
[21]
Wolfe, B.L., et al. (1993) Insulin-Like Growth Factor-I Regulates Transcription of the Elastin Gene. Journal of Biological Chemistry, 268, 12418-12426.
[22]
Qa’aty, N., Vincent, M., Wang, Y., Wang, A., Mitts, T.F. and Hinek, A. (2015) Synthetic Ligands of the Elastin Receptor Induce Elastogenesis in Human Dermal Fibroblasts via Activation of Their IGF-1 Receptors. Journal of Dermatological Science, 80, 175-185. https://doi.org/10.1016/j.jdermsci.2015.10.001
[23]
Kakudo, N., Morimoto, N., Ogawa, T. and Kusumoto, K. (2019) Effects of Fibroblast Growth Factor-2 Combined with a Collagen/Gelatin Sponge for Adipogenesis in the Mouse Subcutis. Annals of Plastic Surgery, 84, 216-221. https://doi.org/10.1097/SAP.0000000000002046
[24]
Li, Z., et al. (2018) Sustained Release of Basic Fibroblast Growth Factor Using Gelatin Hydrogel Improved Left Ventricular Function through the Alteration of Collagen Subtype in a Rat Chronic Myocardial Infarction Model. General Thoracic and Cardiovascular Surgery, 66, 641-647. https://doi.org/10.1007/s11748-018-0969-z
[25]
Fujihara, C., et al. (2019) Fibroblast Growth Factor-2 Inhibits CD40-Mediated Periodontal Inflammation. Journal of Cellular Physiology, 234, 7149-7160. https://doi.org/10.1002/jcp.27469
[26]
Roye, O., et al. (1998) Dermal Endothelial Cells and Keratinocytes Produce IL-7 in Vivo after Human Schistosoma mansoni Percutaneous Infection. The Journal of Immunology, 161, 4161-4168.
[27]
Heufler, C., et al. (1993) Interleukin 7 Is Produced by Murine and Human Keratinocytes. Journal of Experimental Medicine, 178, 1109-1114. https://doi.org/10.1084/jem.178.3.1109
[28]
Bartlett, A., Sanders, A.J., Ruge, F., Harding, K.G. and Jiang, W.G. (2016) Potential Implications of Interleukin-7 in Chronic Wound Healing. Experimental and Therapeutic Medicine, 12, 33-40. https://doi.org/10.3892/etm.2016.3263
[29]
Barata, J.T., Durum, S.K. and Seddon, B. (2019) Flip the Coin: IL-7 and IL-7R in Health and Disease. Nature Immunology, 20, 1584-1593. https://doi.org/10.1038/s41590-019-0479-x
[30]
Phetcharat, L., Wongsuphasawat, K. and Winther, K. (2015) The Effectiveness of a Standardized Rose Hip Powder, Containing Seeds and Shells of Rosa canina, on Cell Longevity, Skin Wrinkles, Moisture, and Elasticity. Clinical Interventions in Aging, 10, 1849-1856. https://doi.org/10.2147/CIA.S90092
[31]
Couteau, C., Coiffard, L.J.M. and Sébille-Rivain, V. (2006) Influence of Excipients on Moisturizing Effect of Urea. Drug Development and Industrial Pharmacy, 32, 239-242. https://doi.org/10.1080/03639040500466361
[32]
3.6.1 R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna. https://www.R-project.org
[33]
Hahn, H.J., et al. (2016) Instrumental Evaluation of Anti-Aging Effects of Cosmetic Formulations Containing Palmitoyl Peptides, Silybum marianum Seed Oil, Vitamin E and Other Functional Ingredients on Aged Human Skin. Experimental and Therapeutic Medicine, 12, 1171-1176. https://doi.org/10.3892/etm.2016.3447
[34]
Lee, D.E., et al. (2015) Clinical Evidence of Effects of Lactobacillus plantarum HY7714 on Skin Aging: A Randomized, Double Blind, Placebo-Controlled Study. Journal of Microbiology and Biotechnology, 25, 2160-2168. https://doi.org/10.4014/jmb.1509.09021
[35]
Barone, F., Bashey, S. and Woodin Jr., F.W. (2019) Clinical Evidence of Dermal and Epidermal Restructuring from a Biologically Active Growth Factor Serum for Skin Rejuvenation. Journal of Drugs in Dermatology, 18, 290-295.
[36]
Bogdan, C., Iurian, S., Tomuta, I. and Moldovan, M. (2017) Improvement of Skin Condition in Striae Distensae: Development, Characterization and Clinical Efficacy of a Cosmetic Product Containing Punica granatum Seed Oil and Croton lechleri Resin Extract. Drug Design, Development and Therapy, 11, 521-531. https://doi.org/10.2147/DDDT.S128470
[37]
Draelos, Z.D., Levy, S.B., Lutrario, C. and Gunt, H. (2018) Evaluation of the Performance of a Nature-Based Sensitive Skin Regimen in Subjects with Clinically Diagnosed Sensitive Skin. Journal of Drugs in Dermatology, 17, 908-913.
[38]
Kwon, H.S., Lee, J.H., Kim, G.M. and Bae, J.M. (2018) Efficacy and Safety of Retinaldehyde 0.1% and 0.05% Creams Used to Treat Photoaged Skin: A Randomized Double-Blind Controlled Trial. Journal of Cosmetic Dermatology, 17, 471-476. https://doi.org/10.1111/jocd.12551
