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中国不同产地薤白ITS、rps16和trnL-F序列分析及亲缘关系分析
ITS, rps16 and trnL-F Sequence Analysis and Genetic Relationships of Alliummacrostemon from Different Regions in China

DOI: 10.12677/BR.2020.94039, PP. 320-327

Keywords: 薤白，ITS，rps16，trnL-F，亲缘关系
Allium macrostemon Bunge, ITS, rps16, trnL-F, Relationship

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Abstract:

本文为探讨不同产地薤白(Allium macrostemon Bunge)的亲缘关系，以40个来自我国不同产地的薤白为材料，分别以ITS、rps16及trnL-F序列进行扩增并测序，分析其序列特征及亲缘关系。结果显示：我国不同产地薤白ITS序列平均长度为590 bp，G + C平均含量为50.64%；rps16序列平均长度为587 bp，G + C平均含量为29.66%；trnL-F序列平均长度为622 bp，G + C平均含量为38.16%。基于ITS + rps16 + trnL-F联合序列构的最大简约法(maximum parsimony, MP)和贝叶斯(Bayesian inference, BI)系统发育树均表明，薤白为一单系种，总体可划分为位于西南地区的A组、沿海地区的B组和北方及东北的C组。研究表明，亲缘关系较近的居群，地理位置也较近，说明ITS + rps16 + trnL-F联合序列可用于不同产地薤白的亲缘关系研究中，可以提供亲缘关系图谱，为薤白资源的开发、保护、利用及育种提供一定的科学资料。
40 Allium macrostemon Bunge individuals from different regions in China were used as materials to explore the genetic relationship. Their sequences of ITS, rps16 and trnL-F were amplified and sequenced respectively, and their sequence characteristics and genetic relationship were analyzed. Results showed that the average length of ITS, rps16 and trnL-F sequences was 590 bp, 587 bp and 622 bp respectively; the average content of G + C was 50.64%, 29.66% and 38.16% respectively. Based on ITS + rps16 + trnL-F sequence structure, the maximum parsimony (MP) and Bayesian inference (BI) phylogenetic trees show that A. macrostemon is a monophyletic species, which can be generally divided into A group in Southwest China, B group in coastal area and C group in North and Northeast China. Research suggested that the populations with close genetic relationships and geographical location were also close, which indicated that ITS + rps16 + trnL-F sequence could be used in the study of genetic relationship of A. macrostemon from different habitats. Based on ITS + rps16 + trnL-F sequence of A. macrostemon, resources from different habitats to construct phylogenetic tree could provide the genetic map and some scientific data for the development, protection, utilization and breeding of A. macrostemon resources.

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