全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用：99美元
投递稿件

查看量下载量

相关文章

更多...

用于研究戊型肝炎病毒的细胞培养系统
Cell Culture System for the Study of the Hepatitis E Virus

DOI: 10.12677/HJBM.2020.103009, PP. 62-67

Keywords: 戊型肝炎病毒，体外复制，细胞培养系统
Hepatitis E Virus, Replication in Vitro, Cell Culture System

Full-Text   Cite this paper   Add to My Lib

Abstract:

戊型肝炎病毒(HEV)是人类戊型肝炎的致病因子，也是全世界肠道传播的病毒性肝炎的主要原因。病毒学研究需要强大的细胞培养系统。不幸的是，与其他肝炎病毒一样，HEV难以在常规细胞中繁殖。虽然已经使用各种HEV毒株测试了许多不同的细胞培养系统，但是病毒复制通常进展非常缓慢，并且具有低病毒粒子计数的感染导致HEV复制无效。然而，最近发现在不同细胞系中传代原发患者分离株的进展改善了体外HEV传播。本综述描述了几个典型毒株的细胞培养情况。
Hepatitis E virus (HEV) is the causative agent of human hepatitis E and the main cause of viral hepatitis transmitted in the intestines worldwide. Virology research requires a powerful cell culture system. Unfortunately, like other hepatitis viruses, HEV is difficult to reproduce in conventional cells. Many different cell culture systems have been tested using various HEV strains, but virus replication generally progresses very slowly, and infections with low virion counts render HEV replication ineffective. However, recent advances in the passage of primary patient isolates in different cell lines have improved in vitro HEV transmission. This review describes the cell culture of several typical strains.

References

[1]  Wedemeyer, H., Pischke, S. and Manns, M.P. (2012) Pathogenesis and Treatment of Hepatitis E Virus Infection. Gastroenterology, 142, 1388-1397e1.
https://doi.org/10.1053/j.gastro.2012.02.014
[2]  Ding, Q., et al. (2017) Hepatitis E Virus ORF3 Is a Functional Ion Channel Required for Release of Infectious Particles. Proceedings of the National Academy of Sciences of the United States of America, 114, 1147-1152.
https://doi.org/10.1073/pnas.1614955114
[3]  Khuroo, M.S. and Kamili, S. (2003) Aetiology, Clinical Course and Outcome of Sporadic Acute Viral Hepatitis in Pregnancy. Journal of Viral Hepatitis, 10, 61-69.
https://doi.org/10.1046/j.1365-2893.2003.00398.x
[4]  Kumar, A., et al. (2004) Hepatitis E in Pregnancy. International Journal of Gynecology & Obstetrics, 85, 240-244.
https://doi.org/10.1016/j.ijgo.2003.11.018
[5]  Smith, D.B., et al. (2016) Proposed Reference Sequences for Hepatitis E Virus Subtypes. Journal of General Virology, 97, 537-542.
https://doi.org/10.1099/jgv.0.000393
[6]  Chen, X., et al. (2012) Recombination and Natural Selection in Hepatitis E Virus Genotypes. Journal of Medical Virology, 84, 1396-407.
https://doi.org/10.1002/jmv.23237
[7]  Huang, F.F., et al. (2004) Determination and Analysis of the Complete Genomic Sequence of Avian Hepatitis E Virus (Avian HEV) and Attempts to Infect Rhesus Monkeys with Avian HEV. Journal of General Virology, 85, 1609-1618.
https://doi.org/10.1099/vir.0.79841-0
[8]  Huang, F., et al. (2009) Experimental Infection of Balb/c Nude Mice with Hepatitis E Virus. BMC Infectious Diseases, 9, 93.
https://doi.org/10.1186/1471-2334-9-93
[9]  Smith, D.B., et al. (2014) Consensus Proposals for Classification of the Family Hepeviridae. Journal of General Virology, 95, 2223-2232.
https://doi.org/10.1099/vir.0.068429-0
[10]  Khuroo, M.S., Khuroo, M.S. and Khuroo, N.S. (2016) Hepatitis E: Discovery, Global Impact, Control and Cure. World Journal of Gastroenterology, 22, 7030-7045.
https://doi.org/10.3748/wjg.v22.i31.7030
[11]  Goens, S.D. and Perdue, M.L. (2004) Hepatitis E Viruses in Humans and Animals. Animal Health Research Reviews, 5, 145-156.
https://doi.org/10.1079/AHR200495
[12]  Tomiyama, D., et al. (2009) Serological Evidence of Infection with Hepatitis E Virus among Wild Yezo-Deer, Cervus nippon yesoensis, in Hokkaido, Japan. Journal of Viral Hepatitis, 16, 524-528.
https://doi.org/10.1111/j.1365-2893.2009.01107.x
[13]  Lee, G.H., et al. (2016) Chronic Infection with Camelid Hepatitis E Virus in a Liver Transplant Recipient Who Regularly Consumes Camel Meat and Milk. Gastroenterology, 150, 355-357.e3.
https://doi.org/10.1053/j.gastro.2015.10.048
[14]  Tei, S., et al. (2003) Zoonotic Transmission of Hepatitis E Virus from Deer to Human Beings. The Lancet, 362, 371-373.
https://doi.org/10.1016/S0140-6736(03)14025-1
[15]  Colson, P., et al. (2010) Pig Liver Sausage as a Source of Hepatitis E Virus Transmission to Humans. The Journal of Infectious Diseases, 202, 825-834.
https://doi.org/10.1086/655898
[16]  Takahashi, M., et al. (2011) Analysis of the Full-Length Genome of a Hepatitis E Virus Isolate Obtained from a Wild Boar in Japan That Is Classifiable into a Novel Genotype. Journal of General Virology, 92, 902-908.
https://doi.org/10.1099/vir.0.029470-0
[17]  Johne, R., et al. (2014) An ORF1-Rearranged Hepatitis E Virus Derived from a Chronically Infected Patient Efficiently Replicates in Cell Culture. Journal of Viral Hepatitis, 21, 447-456.
https://doi.org/10.1111/jvh.12157
[18]  Meng, J., Dubreuil, P. and Pillot, J. (1997) A New PCR-Based Seroneutralization Assay in Cell Culture for Diagnosis of Hepatitis E. Journal of Clinical Microbiology, 35, 1373-1377.
https://doi.org/10.1128/JCM.35.6.1373-1377.1997
[19]  Huang, R., et al. (1995) Hepatitis E Virus (87A Strain) Propagated in A549 Cells. Journal of Medical Virology, 47, 299-302.
https://doi.org/10.1002/jmv.1890470402
[20]  Takahashi, H., et al. (2012) A549 and PLC/PRF/5 Cells Can Support the Efficient Propagation of Swine and Wild Boar Hepatitis E Virus (HEV) Strains: Demonstration of HEV Infectivity of Porcine Liver Sold as Food. Archives of Virology, 157, 235-246.
https://doi.org/10.1007/s00705-011-1153-2
[21]  Wei, S., et al. (2000) 93G, a Novel Sporadic Strain of Hepatitis E Virus in South China Isolated by Cell Culture. Journal of Medical Virology, 61, 311-318.
https://doi.org/10.1002/1096-9071(200007)61:3<311::AID-JMV5>3.0.CO;2-H
[22]  Huang, R., et al. (1999) Cell Culture of Sporadic Hepatitis E Virus in China. Clinical and Diagnostic Laboratory Immunology, 6, 729-733.
https://doi.org/10.1128/CDLI.6.5.729-733.1999
[23]  Tsarev, S.A., et al. (1992) Characterization of a Prototype Strain of Hepatitis E Virus. Proceedings of the National Academy of Sciences of the United States of America, 89, 559-563.
https://doi.org/10.1073/pnas.89.2.559
[24]  Shukla, P., et al. (2011) Cross-Species Infections of Cultured Cells by Hepatitis E Virus and Discovery of an Infectious Virus-Host Recombinant. Proceedings of the National Academy of Sciences of the United States of America, 108, 2438-2443.
https://doi.org/10.1073/pnas.1018878108
[25]  Shukla, P., et al. (2012) Adaptation of a Genotype 3 Hepatitis E Virus to Efficient Growth in Cell Culture Depends on an Inserted Human Gene Segment Acquired by Recombination. Journal of Virology, 86, 5697-5707.
https://doi.org/10.1128/JVI.00146-12
[26]  Yu, W., et al. (2014) Complete Genome Sequence of Swine Hepatitis E Virus Prevalent in Southwest China. Genome Announcements, 2, e00090-14.
https://doi.org/10.1128/genomeA.00090-14
[27]  Li, Y., et al. (2016) Construction of Recombinant Full-Length Hepatitis E Virus Fused with EGFP and Assessment of Infectivity. Chinese Journal of Virology, 32, 529-537.
[28]  Huang, F., et al. (2016) Hepatitis E Virus Infection Activates Signal Regulator Protein α to Down-Regulate Type I Interferon. Immunologic Research, 64, 115-122.
https://doi.org/10.1007/s12026-015-8729-y
[29]  Bi, Y., et al. (2015) Pregnancy Serum Facilitates Hepatitis E Virus Replication in Vitro. Journal of General Virology, 96, 1055-1061.
https://doi.org/10.1099/vir.0.000054
[30]  曹文涛, 郝先辉, 赵勇琴, 司徒健文, 何秋霞, 纪汉斌, 杨伟敏, 黄芬. 天然免疫抗病毒因子RIG-I过表达对HEV复制的抑制作用[J]. 昆明理工大学学报(自然科学版), 2019, 44(3): 86-92.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133