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肠道气泡堆积对银鲳四种组织代谢酶活力的影响
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Abstract:
肠道气泡堆积是银鲳养殖过程中的常见病，具有发病快，死亡率高的特点，严重阻碍银鲳的规模养殖及推广。为研究肠道气泡堆积对银鲳组织代谢酶活力的影响，2019年3月于东海水产研究所福鼎研究中心采集了15尾肠道气泡堆积的银鲳为病鱼组(11.7 ± 0.8 cm, 42.8 ± 4.1 g)，15尾健康银鲳为对照组(健康鱼组) (12.5 ± 0.7 cm, 48.6 ± 5.6 g)，分别采集各组样品的肝脏，肾脏，肌肉和鳃组织，并对这四种组织谷草转氨酶活力(AST)、谷丙转氨酶活力(ALT)、碱性磷酸酶(ALP)和酸性磷酸酶活力(ACP)活力进行测定和比较，实验结果显示：病鱼组肌肉和肾脏中AST活力显著低于健康组(P < 0.05)；病鱼组肝脏和肌肉中的ALT活力显著低于健康组(P < 0.05)；两组样品四种组织中ALP活力均有显著差异；病鱼组肝脏中ACP活力显著高于健康组(P < 0.05)，但在鳃和肾脏中显著低于健康组(P < 0.05)。研究结果表明，肠道气泡堆积会导致银鲳组织代谢紊乱，且对银鲳肌肉ALT，肾脏ALP活力影响较大，这两种酶活力指标可以成为该病检测和病理分析的重要参考依据。
Intestinal gas bubble accumulation is a common disease in the breeding process of silver pomfret, which is characterized by fast onset and high mortality, and seriously hinders the large-scale farm-ing and promotion of silver pomfret. In order to investigate the effect of intestinal gas bubble accu-mulation on the metabolizing enzyme activity of silver pomfret, fifteen diseased fish were chosen as group diseased (11.7 ± 0.8 cm, 42.8 ± 4.1 g) and fifteen healthy fish as group healthy (12.5 ± 0.7 cm, 48.6 ± 5.6 g). These fish were collected from Fuding research center of East China Sea Fisheries Re-search Institute in March 2019. Liver, kidney, muscle, and gill tissues of each group were collected and used to test their activities of AST, ALT, ALP, and ACP. The results showed that: the activities of in muscle and kidney in group diseased were significantly lower than those in group healthy (P < 0.05); the activities of ALT in liver and muscle in group diseased were significantly lower than those in group healthy (P < 0.05); the activities of ALP in four tissues of the two groups were significantly different; the activity of ACP in liver in group diseased were significantly lower than those in group healthy (P < 0.05), but in gill and kidney, it was significantly lower than those in group healthy (P < 0.05). The results showed that the intestinal gas bubble accumulation would lead to the disorder of tissue metabolism of silver pomfret, and had a great influence on the activity of ALT in muscle and ALP in kidney of silver pomfret. These two indexes of enzyme activity could be an important refer-ence for the detection and pathological analysis of the disease.
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