基于数字全息显微的牛奶中样本成像方法的研究
Study on the Imaging Method of Samples Immersed in Milk Based on Digital Holographic Microscopy

光透过牛奶介质时，散射作用使牛奶中样本成像模糊。数字全息显微技术通过数值衍射计算重构物光波前的强度与相位，并通过数字滤波技术降低散射作用引入的噪声。本文利用透射式数字全息显微系统对牛奶中的分辨率样板进行成像，并讨论入射光强对成像的影响作用。实验表明，在入射光源弱至完全不能对牛奶中样本成像的情况下，利用数字全息显微技术仍然能够获取清晰的样本重构强度像，因此，数字全息显微是一种适用于牛奶中样本成像的技术手段。
The imaging of samples immersed in milk media is blurred because of the scattering effect. By utilizing the digital holography technique, the phase and intensity of wave front of the transmitting beam through the non-transparent media can be reconstructed, while the noises introduced by the scattering material can be filtered by digital filtering algorithm. In this paper, the transmission digital holographic microscopic system was applied in imaging of USAF resolution plate immersed in milk. The effect of incident light intensity on imaging is also discussed. The experiment results show that even when the incident light source is too weak to image the sample in milk, by applying digital holographic microscopy, clear reconstructed intensity image of sample in milk can still be obtained. Therefore, digital holography technique is especially suitable for the imaging of samples immersed in milk media.