烟草马铃薯X病毒(Potatoes Virus X, PVX)快速检测方法及抗性诱导物质抑制效果研究 Rapid Detection Method of Tobacco Potato Virus X (PVX) and Study on the Inhibitory Effect of Resistance Inducer
对注射PVX后烟苗的发病症状；总RNA快速检测PVX侵染烟草叶片的方法；注射抗病毒抗性诱导物质处理后周围病斑扩散情况；摩擦法接种抗性诱导物质效果等方面进行了研究。结果表明：注射1周后观察云烟叶片，PVX在云烟中可产生严重的花叶症状，PVX在云烟中表达成功；提取RNA，利用病毒的引物检测PVX病毒的纯度，经检测RNA OD260/280 = 1.955，C = 755.89 ng/μL，说明发病叶片是由纯的PVX引起；注射1 d后用抗性诱导物质比施壮处理叶片，2 d后利用UV光照射并拍照，发现经比施壮处理后，病毒扩散斑明显小于非施用处理；在抗性诱导物质胁迫下，病毒复制效率运动扩散能力都有所降低，抗性诱导物质处理后的叶片荧光斑虽有所扩大，但病毒尚未发生明显转移。由此可见：提取侵染烟草叶片总RNA进行PVX纯度检测，可灵敏地检测出马铃薯X病毒，可作为快速评价感染马铃薯X病毒的指标。抗性诱导物质(比施壮)可抑制马铃薯X病毒的扩散。
The symptoms of tobacco seedlings after injection of PVX, the method of rapid detection of total RNA PVX infection of tobacco leaves, the diffusion of surrounding spots after treatment with antiviral resistance inducers, and the effect of friction inoculation resistance inducers were studied. The results showed that PVX could produce serious mosaic symptom in tobacco leaves after one week of injection; PVX was successfully expressed in tobacco leaves; RNA was extracted; the purity of PVX virus was detected by using the primers of virus; and the RNA OD260/280 = 1.955, c = 755.89 ng/μL was detected. The results showed that the infected leaves were caused by pure PVX. The virus spreading spot was smaller after 1 d treatment than that after 2 d treatment by UV irradiation and photo-taking. Under the stress of resistance inducer, the virus replication efficiency, movement and diffusion ability were all decreased, and the fluorescence spot of leaves treated by resistance inducer was enlarged, but the virus had not been transferred obviously. The results showed that PVX virus could be detected sensitively by extracting total RNA from tobacco leaves, which could be used as a rapid index to evaluate the infection of PVX virus. The resistance inducer (Bz) could inhibit the spread of potato X virus.
