广西虾源副溶血弧菌喹诺酮类耐药基因qnrC、qnrS、qnrVC的检测及分析
Detection and Analysis of Quinolone Resistance Genes, qnrC, qnrS and qnrVC, in Vibrio Parahaemolyticus from Shrimp in Guangxi

本实验通过PCR方法检测34株广西凡纳滨对虾源副溶血弧菌中喹诺酮类耐药基因qnrC、qnrS和qnrVC的分布情况。耐药基因检测结果表明，34株副溶血弧菌中qnrC、qnrS和qnrVC基因的检出率分别为2.9%、0.0%与2.9%。qnrC基因在防城港市、钦州市、北海市的检出率分别为16.7%、0%、0%。qnrS基因在防城港市、钦州市、北海市的检出率均为0%。qnrVC基因在防城港市、钦州市、北海市的检出率分别为0%、0%、6.3%，三种耐药基因的检出率在三个市的副溶血弧菌之间差异均不显著(P > 0.05)。基于qnrC基因的氨基酸序列同源性比较结果表明，防城港株F17383与副溶血弧菌TOE26912.1、TOP53441.1、OQU02262.1亲缘关系最近，氨基酸同源性分别为100.0%、98.8%和98.8%。基于qnrVC基因氨基酸序列同源性比较结果表明，北海株B13121与副溶血弧菌AXI69764.1亲缘关系最近，同源性为99.4%。
In this study, the distribution of quinolone resistance genes qnrC, qnrS, and qnrVC in 34 strains of Vibrio parahaemolyticus from shrimp in Guangxi was detected by PCR. The detection results of antibiotic resistance genes showed that the detection rates of qnrC, qnrS and qnrVC genes in 34 strains of V. parahaemolyticus are 2.9%, 0.0% and 2.9% respectively. The detection rates of qnrC gene in Fangchenggang City, Qinzhou City and Beihai City were 16.7%, 0% and 0%, respectively. The detection rates of qnrS gene in Fangchenggang City, Qinzhou City and Beihai City were all 0%. The detection rates of qnrVC gene in Fangchenggang City, Qinzhou City and Beihai City were 0%, 0% and 6.3%, respectively. The detection rates of the three antibiotic resistance genes were not significantly different among the strains of Vibrio parahaemolyticus of three cities (P > 0.05). Based on the homology comparison of the amino acid sequences of qnrC gene, the Fangchenggang strain F17383 had the closest genetic relationship with V. parahaemolyticus TOE26912.1, TOP53441.1 and OQU02262.1, sharing homology of 100.0%, 98.8% and 98.8%, respectively. And based on the homology comparison of the amino acid sequences of qnrVC gene, the Beihai strain B13121 had the closest genetic relationship with V. parahaemolyticus AXI69764.1, sharing homology of 99.4%.