雌性克氏原螯虾不同性腺发育时期肝胰腺生化组成变化
Biochemical Composition Changes of Hepatopancreas during Gonadal Development of Female Crayfish Procambarus clarkia

本实验研究了克氏原螯虾不同发育时期肝胰腺指数变化情况，并比较了肝胰腺中蛋白、总脂、总糖、水分及脂肪酸组成变化，结果表明，1) 克氏原螯虾肝胰腺指数在性腺发育处于六期时(排卵后)和一期时最高，随着性腺发育逐步成熟，雌性克氏原螯虾肝胰腺指数持续下降，此时期也是崇明地区克氏原螯虾的繁殖高峰，性腺发育处于四期时，肝胰腺指数达到最低值。2) 随着性腺的发育，肝胰腺中水分含量逐渐升高，肝胰腺的干物质中，蛋白、总糖含量均随着性腺发育显著下降。3) 肝胰腺脂肪酸组成上，C16:0随着性腺发育逐渐减少，C16:1n7和C18:3n3随着性腺发育先增加后减少，产卵后达到最低，而C18:2n6随着性腺发育逐渐增加。4) 三种高不饱和脂肪酸C20:4n6 (花生四烯酸ARA)，C20:5n3 (EPA)，C22:6n3 (DHA)均呈显著减少趋势。
This study investigated the variation of hepatopancreas index (HSI), as well as hepatosomatic moisture, lipid, protein, carbo and fat acid contents, of female crayfish (Procambarus clarkia) during the ovarian development period. The results showed that: 1) The decreasing trend was observed on HSI with the development of P. clarkia ovarian from Stage I to Stage IV, but further ovarian development from Stage V to Stage VI increased the HSI. 2) Hepatosomatic moisture showed a slightly increasing trend with the ovarian development, but hepatosomatic protein and carbo contents showed markedly deceasing trends with ovarian development. 3) As for fat acid composition, C16:0 and C18:2n6 respectively showed a slightly decreasing and increasing trend with ovarian development, in addition, C16:1n7 and C18:3n3 both showed an increasing trend with ovarian development from Stage I to Stage IV, but leveled off thereafter. 4) C20:4n6 (ARA), C20:5n3 (EPA) and C22:6n3 (DHA) all showed a decreasing trend with ovarian development.