A preclinical ultrasound method for the assessment of vascular disease progression in murine models

The efficacy of preclinical ultrasound at providing a quantitative assessment of mouse models of vascular disease is relatively unknown. In this study, preclinical ultrasound was used in combination with a semi-automatic image processing method to track arterial distension alterations in mouse models of abdominal aortic aneurysm and atherosclerosis. Longitudinal B-mode ultrasound images of the abdominal aorta were acquired using a preclinical ultrasound scanner. Arterial distension was assessed using a semi-automatic image processing algorithm to track vessel wall motion over the cardiac cycle. A standard, manual analysis method was applied for comparison. Mean arterial distension was significantly lower in abdominal aortic aneurysm mice between day 0 and day 7 post-onset of disease (p？<？0.01) and between day 0 and day 14 (p？<？0.001), while no difference was observed in sham control mice. Manual analysis detected a significant decrease (p？<？0.05) between day 0 and day 14 only. Atherosclerotic mice showed alterations in arterial distension relating to genetic modification and diet. Arterial distension was significantly lower (p？<？0.05) in Ldlr？/？ (++/？？) mice fed high-fat western diet when compared with both wild type (++/++) mice and Ldlr？/？ (++/？？) mice fed chow diet. The manual method did not detect a significant difference between these groups. Arterial distension can be used as an early marker for the detection of arterial disease in murine models. The semi-automatic analysis method provided increased sensitivity to differences between experimental groups when compared to the manual analysis method